人胞內(nèi)細(xì)胞因子檢測(cè)刺激方案
 |
| 人細(xì)胞因子 | 細(xì)胞來(lái)源 | 活化-極化培養(yǎng) | 培養(yǎng)時(shí)間 | 再次刺激 | 胞內(nèi)阻斷劑 |
| G-CSF | PBMC | LPS (1 ug/mL) | 24hr | - | Monensin |
| GM-CSF | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 5hr | - | Monensin |
| GIFN gamma | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 5hr | - | Brefeldin A |
| IL-1 alpha | PBMC | LPS (1 ug/mL) | 24hr | - | Monensin |
| IL-1 beta | PBMC | LPS (100ng/mL) | 4hr | - | Brefeldin A |
| IL-1RA | PBMC | LPS (100ng/mL) | 24hr | - | Brefeldin A |
| IL-2 | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 4-6hr | - | Brefeldin A |
| IL-4 | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 4-6hr | - | Brefeldin A |
| IL-5 | CD4 | Th2 polarizing cultures | 6d | PMA (50ng/mL) + Ionomycin (1ug/mL) (5hr) | Brefeldin A |
| IL-6 | PBMC | LPS (100ng/mL) | 24hr | - | Brefeldin A |
| IL-9 | CD4 | h2 polarizing culturesT | 6d | PMA (50ng/mL) + Ionomycin (1ug/mL) (5hr) | Monensin |
| IL-10 | CD4 | Th2 polarizing cultures | 6d | PMA (50ng/ml) + Ionomycin (1ug/ml) (5hr) | Monensin |
| IL-12/ IL-23 (p40) | PBMC |
hIFN gamma (100ng/mL) (2hr)/LPS (100ng/mL) (22hr) | 2hr/22hr | - | Brefeldin A |
| IL-13 | CD4 | anti-CD3 (10ug/mL, immobilized) + anti-CD28 (2ug/mL, soluble) + IL-2 (10ng/mL) + IL-4 (20ng/mL) (2d); IL-2 (10ng/mL) + IL-4 (20ng/mL) (3d) | 2d/3d | PMA (5ng/ml) + Ionomycin (500ng/ml) (4hr) | Brefeldin A |
| IL-17A | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 5hr | - | Monensin |
| IL-21 | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 4-7hr or 12-18hr | - | Brefeldin A |
| IL-22 | CD4 | Th17 polarizing cultures | 6d | PMA (50ng/ml) + Ionomycin (1ug/ml) (5hr) | Brefeldin A |
| IL-23 p19 | PBMC | hGM-CSF (40ng/mL) +hIL-4 (40ng/mL) | 6d | LPS (1ug/mL) (24hr) | Monensin |
| MCP-1/ CCL2 | PBMC | LPS (1ug/mL) | 24hr | - | Monensin |
| RANTES/ CCL5 | PBMC | LPS (1ug/mL) | 24hr | - | Monensin |
| TNF alpha | PBMC | PMA (30-50ng/mL)/Iono (1ug/mL) | 5hr | - | Brefeldin A |
| TNF beta | PBMC | Th1 polarizing cultures | 6d | PMA (50ng/mL) + Ionomycin (1ug/mL) (5hr) | Monensin |
|
注： PBMC=外周血單核細(xì)胞peripheral blood mononuclear cell；PMA=佛波酯Phorbol Myristate Acetate; calcium ionophore; 鈣離子載體 iono=離子霉素ionomycin; LPS=脂多糖Lipopolysaccharide; 4hr=4 hours culture; 2d=2 days culture. GolgiStop/GolgiPlug: 一種胞內(nèi)阻斷劑，可以用BFA/Monensin替代。
| |||||
 |
閱讀原文：
- 培養(yǎng)肉從細(xì)胞培養(yǎng)到生產(chǎn)的關(guān)鍵步驟及工藝流程
- CAR-T細(xì)胞的開(kāi)關(guān)研究助力提高療法的安全性和有效性
- 小鼠膽管癌細(xì)胞LD-1培養(yǎng)步驟說(shuō)明書(shū)
- 全球首個(gè)間充質(zhì)干細(xì)胞療法FDA獲批上市審批歷程揭秘
- 各類(lèi)免疫細(xì)胞表型分類(lèi)、特點(diǎn)及功能
- γδ T 細(xì)胞的基本特性、常用擴(kuò)增培養(yǎng)方法及原理
- 細(xì)胞培養(yǎng)全流程中使用的消化液、培養(yǎng)基、細(xì)胞因子及作用介紹
- 案例分享：T細(xì)胞銜接劑TCE和CAR-T在AID臨床治療中的有效性對(duì)比
- 中喬新舟限時(shí)促銷(xiāo)：買(mǎi)細(xì)胞就送支原體檢測(cè)試劑盒
- 華威中儀試劑盒年末限時(shí)優(yōu)惠來(lái)襲，儲(chǔ)值享好禮
- 美德聲推出新品重組細(xì)胞系用于生產(chǎn)單克隆血型試劑
- 百螢小麥胚芽凝集素探針染料8折優(yōu)惠，買(mǎi)即贈(zèng)鼠標(biāo)墊
- 上海惠誠(chéng)生物成為安普未來(lái)恒溫?cái)U(kuò)增試劑盒代理商
- 雙11科研狂歡促銷(xiāo)，逐典攜手埃澤思下單滿(mǎn)額雙重好禮
- MCE 第三屆細(xì)胞攝影大賽圓滿(mǎn)落幕，評(píng)選結(jié)果公布
- MCE教師節(jié)直播:細(xì)胞凋亡的機(jī)制、標(biāo)志物與檢測(cè)方法
Copyright(C) 1998-2024 生物器材網(wǎng) 電話(huà)：021-64166852;13621656896 E-mail：info@bio-equip.com