DYKDDDDK Fab-Trap™ Agarose是高效免疫沉淀(IP)Flag®標記蛋白質的高級親和樹脂。DYKDDDDK Fab-Trap™ Agarose由一個Fab片段組成,該片段與瓊脂糖珠共價結合。
應用
Immunoprecipitation (IP) / Co-IP 免疫沉淀(IP)/共沉淀
Immunoprecipitation (IP) / Co-IP質譜(MS)
Protein purification 蛋白質純化
ChIP/ RIP analysis ChIP/RIP分析
On-bead enzyme assays 微珠酶分析
實驗結果
DYKDDDDK Fab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide (fp-1) the enriched Flag® fusion protein is released from DYKDDDDK Fab-Trap™. Western blot was probed with DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (Proteintech, 20543-1-AP) and Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] (srbAF488-1). L: Prestained protein marker (Proteintech, PL00001), I: Input, FT: Flow-Through, E: Elution.
訂貨信息
DYKDDDDK Immunoprecipitation Starter Pack
DYKDDDDK Fab-Trap™ Agarose (20 reactions, product code ffa-20) for immunoprecipitation, 3xDYKDDDDK-peptide (1mg, fp-1) for elution and DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20UL) for detection in Western blots.
Starter Pack (ffap) 包括以下組分:
· DYKDDDDK Fab-Trap™ Agarose (20 reactions, ffa-20),
· 3xDYKDDDDK-peptide (1mg, fp-1) and
· DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20).
默瑞生物
默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學產品推廣與服務商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務體系,提升客戶體驗,結合國內外優(yōu)質產品提供高標準完整解決方案,幫助科研客戶和生物技術企業(yè)高效創(chuàng)新,加速轉化,創(chuàng)造更多優(yōu)質產品,提升社會福祉,實現(xiàn)多方共贏。
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