關(guān)于ZL-23010 – ZL-23078 的輔酶A系列產(chǎn)品，國(guó)外建議如下：
輔酶A衍生物不建議使用普通的RP C18色譜柱進(jìn)行分析，因這類化合物會(huì)粘附在色譜柱上，難以從色譜柱中洗脫出來。
色譜條件建議如下：
Chromolith Type色譜柱
洗脫劑：10％溶劑B（乙腈/甲醇）+ 90％溶劑A（0.1％甲酸水溶液）
若您選取的是普通的C18色譜柱，溶劑A務(wù)必調(diào)整為0.2% 三氟乙酸(TFA) 水溶液即每1L水中三氟乙酸用量2mL (溶劑A)。
梯度洗脫   溶劑A：0.2％TFA水溶液   溶劑B：乙腈/甲醇 
                    0 - 8 min     A：B = 80：20
    8 - 9 min      B = 100%
                    9 - 10 min    A：B = 80：20
 
國(guó)外關(guān)于該類產(chǎn)品的原文說明如下：
Please tell the customer he should read the Certificate of analysis with attention,
because the Coenzyme-A-Derivatives can NOT be tested with usual, ordinary RP C18 columns, because the compound is stick (adhere) on the column an it is NOT eluting from the column !!!
For getting elution, the customer need to use Chromolith Type columns with 0.1% Formic Acid in Water and starting HPLC-MS conditions with 10% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.1% Formic Acid).
In case of using common RP C18 columns, proceed as following: Use as Solvent A 0.2% TFA (Trifluoroacetic Acid - this is meaning 2ml TFA per Liter Water) and starting HPLC-MS conditions using 20% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.2% Trifluoroacetic Acid) than increase in 8 minutes the Solvent B to 100%, keep 1 minute at 100% and go back to 20% Solvent B in 1 Minute keeping this gradient for another minute.