植物組織培養(yǎng)廣泛應(yīng)用于轉(zhuǎn)基因研究、作物育種、觀賞花卉進(jìn)出口、中藥材生產(chǎn)等領(lǐng)域，但組培過(guò)程中的種苗污染問(wèn)題，尤其是真菌和內(nèi)生菌的污染一直是廣大科研工作者所關(guān)心和頭疼的問(wèn)題。西美杰代理的美國(guó)Plant Cell Technology（簡(jiǎn)稱(chēng)PCT）專(zhuān)門(mén)為植物組織培養(yǎng)而研發(fā)的專(zhuān)利產(chǎn)品——PPM^TM，可有效抑制由于空氣、滅菌不徹底、交叉污染、外植體消毒等各種原因?qū)е碌募?xì)菌、真菌和農(nóng)桿菌污染。PPM^TM是一種熱穩(wěn)定的抗菌劑，在合理的使用劑量下，不會(huì)對(duì)植物生長(zhǎng)產(chǎn)生任何影響。并能廣泛適用于絕大多數(shù)被子植物和裸子植物。

• PPM^TM相對(duì)于傳統(tǒng)抗生素具有以下優(yōu)勢(shì)：
• PPM^TM廣泛抑制和清除細(xì)菌和真菌的污染，并防止真菌孢子的萌發(fā)。
• PPM^TM是通過(guò)抑制多種酶的活性而達(dá)到抑菌效果，從而能避免耐藥突變體的發(fā)生。
• PPM^TM具有熱穩(wěn)定性，無(wú)需過(guò)濾滅菌，能直接加入培養(yǎng)基高溫滅菌而不會(huì)影響使用效果。
• PPM^TM用量低，性價(jià)比高。

PCT自1995年開(kāi)始著力于PPM^TM的研究，迄今為止累積50多篇已發(fā)表文獻(xiàn)，除此之外更有來(lái)自全世界植物研究者使用心得反饋。下表匯總了部分客戶的使用反饋，幫助廣大科研人員更深入了解PPM^TM ，以便有效解決植物污染問(wèn)題。
 

PLANT NAME	C(mol/L)	TISSUE TYPE	RESULT / COMMENTS
Potato	1ml/L	Shoot/Callus	used 1ml/l in our culture media to avoid contaminants
Vitis (Grape)	15mL/L	Nodal Segments;	Helpful to control endophytic bacteria in Vitis shoots taken from potted plants and introduced to tissue culture for several weeks with minimal/no toxic effects
Petunia hybrida	5mL/L	Upper young axillary buds	1. HPLC revealed that axillary buds from upper young nodes absorbed significantly more PPM compared with those from the lower nodes. 2. Indexation indicated successful eradication of bacterial contaminants from upper young axillary buds after vacuum-infiltration with 5 ml l−1 PPM
T. modesta	1mL/L	The bulbs of T. modesta	The contamination was only by fungi because the bacterial contamination could be efficiently controlled with streptomycin and PPM as biocides.
orchid	1mL/L	leaf	Great, five star product!
Citrus	4mL/L	Shoot / Tip	eliminates fungus, does not seem to have effect on plant
Wheat	0.5ml/l	wheat embryo culture	we use in regeneration media and sometimes to kill the fungi in regenerated plants

請(qǐng)點(diǎn)擊下面鏈接查看更多物種PPM^TM使用建議：
https://www.plantcelltechnology.com/how-people-are-using-ppm/

• 關(guān)注公眾號(hào)即可獲取50多篇PPM^TM已發(fā)表文獻(xiàn)

如果您還想了解更多植物學(xué)相關(guān)產(chǎn)品信息，歡迎隨時(shí)撥打PPM中國(guó)一級(jí)代理北京西美杰科技有限公司客服熱線400-050-4006或登錄官網(wǎng)www.xmjsci.com查詢訂購(gòu)。