動(dòng)物術(shù)中和術(shù)后保溫是動(dòng)物實(shí)驗(yàn)過(guò)程中的一個(gè)至關(guān)重要的環(huán)節(jié)，是否做好保溫措施直接關(guān)系到實(shí)驗(yàn)的成敗，同時(shí)也是科研工作者在進(jìn)行生命科學(xué)相關(guān)研究中將實(shí)驗(yàn)動(dòng)物倫理和福利付諸實(shí)踐的真正體現(xiàn)，是對(duì)動(dòng)物愛(ài)心的社會(huì)責(zé)任感的體現(xiàn)。善待活著的動(dòng)物，減少動(dòng)物死亡的痛苦�。�！

動(dòng)物手術(shù)保溫的科學(xué)價(jià)值

Responsible scientists know that good science and good animal care go hand in hand, and would not tolerate cruel or inhumane treatment of any laboratory animal.

------The U.S. Animal Welfare Act

美國(guó)動(dòng)物福利法案申明指出“負(fù)責(zé)的科學(xué)家深知，好科學(xué)與善待動(dòng)物是相輔相成的，我們不能容忍殘殺或不人道的對(duì)待任何實(shí)驗(yàn)動(dòng)物�！�

保障動(dòng)物實(shí)驗(yàn)結(jié)果準(zhǔn)確性：科研效果更好、結(jié)果可信、更加客觀真實(shí)

動(dòng)物手術(shù)低體溫的危害

為什么需要對(duì)手術(shù)動(dòng)物進(jìn)行保溫？

1. 損害機(jī)體免疫功能，減少皮膚血流和氧供，增加傷口感染率

2. 降低血流循環(huán)和血液指標(biāo)，導(dǎo)致凝血發(fā)生，非常不利于血液或血流相關(guān)指標(biāo)監(jiān)測(cè)和測(cè)定，比如測(cè)量血流量、血壓等

3. 降低藥物在機(jī)體組織中的代謝速度，影響機(jī)體代謝

4. 延長(zhǎng)麻醉恢復(fù)時(shí)間，可能明顯增加動(dòng)物死亡率

5. 引起其他疾病的發(fā)生，比如心血管并發(fā)癥

6. 無(wú)法得到真實(shí)或接近真實(shí)的實(shí)驗(yàn)數(shù)據(jù)

7. More………

動(dòng)物手術(shù)低體溫的常見(jiàn)原因

• 1.麻醉劑的作用：麻醉劑導(dǎo)致擴(kuò)張血管、抑制體溫調(diào)節(jié)，從而導(dǎo)致體溫下降

• 2.手術(shù)室的低溫環(huán)境，尤其是普通動(dòng)物室實(shí)驗(yàn)環(huán)境

• 3.手術(shù)臺(tái)的“冷”材質(zhì)，比如不銹鋼、樹(shù)脂材質(zhì)的臺(tái)面

• 4.長(zhǎng)時(shí)間藥物注射：長(zhǎng)時(shí)間輸入與環(huán)境等溫的液體，對(duì)動(dòng)物體液造成“冷稀釋”作用，從而導(dǎo)致動(dòng)物體溫下降

• 5.皮膚消毒劑的使用，尤其是裸露皮膚大面積的消毒，導(dǎo)致體內(nèi)熱量散失

• 長(zhǎng)時(shí)間的手術(shù)

• 6.創(chuàng)傷較大的開(kāi)胸、開(kāi)腹手術(shù)，造成熱量散發(fā)較多

• 7.動(dòng)物自身年齡、疾病因素，如營(yíng)養(yǎng)不良、腫瘤模型手術(shù)

• Others………

動(dòng)物手術(shù)保溫的常見(jiàn)措施

Solution 1

Solution 2

動(dòng)物手術(shù)保溫的規(guī)范操作

術(shù)中

1.如果使用電熱毯保溫方式，切忌電熱毯直接放置于不銹鋼、樹(shù)脂等“冷”材質(zhì)手術(shù)臺(tái)面，因這些材質(zhì)傳熱會(huì)導(dǎo)致電熱毯加熱很慢甚至無(wú)法升溫到預(yù)期溫度；
2.除手術(shù)部位外，其他部位盡量不暴露，可以使用布巾遮蓋；
3.如果是注射液體或藥物，且實(shí)驗(yàn)時(shí)間較長(zhǎng)，必要時(shí)，可以同時(shí)對(duì)輸進(jìn)動(dòng)物體內(nèi)的液體進(jìn)行預(yù)熱；
4.手術(shù)操作配合熟練，減少手術(shù)時(shí)間；
5.注意體溫監(jiān)測(cè)。

術(shù)后

• 繼續(xù)將動(dòng)物放于保溫毯或保溫臺(tái)上，待動(dòng)物恢復(fù)。

動(dòng)物手術(shù)麻醉、保溫全套方案

Solution 1

此套方案集吸入麻醉、立體定位和動(dòng)物手術(shù)保溫全套功能于一體，是從事小鼠、大鼠等小動(dòng)物神經(jīng)科學(xué)相關(guān)研究的標(biāo)準(zhǔn)配置，主要應(yīng)用范圍：

1. 1.光遺傳

2. 2.神經(jīng)電生理

3. 3.鈣成像

4. 4.微透析

5. 5.顱腦&脊髓損傷

6. 6.顱內(nèi)給藥

7. and more……..

Solution 2

此套方案集吸入麻醉、動(dòng)物身體固定和手術(shù)保溫全套功能于一體，是從事小鼠、大鼠等小動(dòng)物常用實(shí)驗(yàn)的標(biāo)準(zhǔn)配置，主要應(yīng)用范圍：

1. 1.動(dòng)物解剖、分離

2. 2.腦梗、心梗等模型造模

3. 3.心臟、尾靜脈、靜脈等缺血

4. 4.頸內(nèi)靜脈和尾靜脈給藥

5. 5.動(dòng)物活體成像

6. and more……..

瑞沃德品牌體溫維持儀引用文獻(xiàn)（部分）：

1. 1.Li L, Tang Y, Sun L, et al. In vivo fiber photometry of neural activity in response to optogenetically manipulated inputs in freely moving mice[J]. Journal of Innovative Optical Health Sciences, 2017: 1743001.

2. 2.Zhu S Z, Gu Y, Wu Z, et al. Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury and augments inflammatory response in rats[J]. Biochemical and biophysical research communications, 2016, 474(1): 175-181.

3. 3.Chernyuk D P, Vlasova O L, Bezprozvanny I B, et al. Hyperexpression of STIM2 protein lowers the amount of Abeta plaques in the brain of Alzheimer's disease mouse model[J]. St. Petersburg Polytechnical University Journal: Physics and Mathematics, 2016, 2(4): 329-336.

4. 4.Xin Y, Li Z, Wu W, et al. Recognition unit-free and self-cleaning photoelectrochemical sensing platform on TiO 2 nanotube photonic crystals for sensitive and selective detection of dopamine release from mouse brain[J]. Biosensors and Bioelectronics, 2017, 87: 396-403.

5. 5.Gu H, Yang Y, Zhou X, et al. Online electrochemical method for continuous and simultaneous monitoring of glucose and l-lactate in vivo with graphene hybrids as the electrocatalyst[J]. Journal of Electroanalytical Chemistry, 2014, 730: 41-47.

6. 6.Huang L, Yuan T, Tan M, et al. A retinoraphe projection regulates serotonergic activity and looming-evoked defensive behaviour[J]. Nature Communications, 2017, 8.

7. 7.Yue X H, Tong J Q, Wang Z J, et al. Steroid sulfatase inhibitor DU-14 protects spatial memory and synaptic plasticity from disruption by amyloid β protein in male rats[J]. Hormones and behavior, 2016, 83: 83-92.

8. 8.Tang R. In vivo imaging of lung tumor growth using in-line x-ray phase contrast technique.(2016) J Imag-ing Sci 1 (1): 1-3[J]. J Imaging Sci, 2016, 1(1).

9. 9.Zhang Q, Lan Y, He X, et al. Allopurinol protects against ischemic insults in a mouse model of cortical microinfarction[J]. Brain research, 2015, 1622: 361-367.

10. 10.Lin X, Miao P, Wang J, et al. Surgery-related thrombosis critically affects the brain infarct volume in mice following transient middle cerebral artery occlusion[J]. PloS one, 2013, 8(9): e75561.

11. 11.Cai J, He C, Chen L, et al. Melatonin mitigate cerebral vasospasm after experimental subarachnoid hemorrhage: a study of synchrotron radiation angiography[J]. Journal of Instrumentation, 2013, 8(06): C06004.

12. 12.Liu T, Zhang T, Yu H, et al. Adjudin protects against cerebral ischemia reperfusion injury by inhibition of neuroinflammation and blood-brain barrier disruption[J]. Journal of neuroinflammation, 2014, 11(1): 107.

13. 13.Pan R, Cai J, Zhan L, et al. Buyang Huanwu decoction facilitates neurorehabilitation through an improvement of synaptic plasticity in cerebral ischemic rats[J]. BMC complementary and alternative medicine, 2017, 17(1): 173.

14. 14.Tang M, Lin W, Pan Y, et al. Hippocampal neurogenesis dysfunction linked to depressive-like behaviors in a neuroinflammation induced model of depression[J]. Physiology & behavior, 2016, 161: 166-173.

15. 15.Yan A, Zhang T, Yang X, et al. Thromboxane A2 receptor antagonist SQ29548 reduces ischemic stroke-induced microglia/macrophages activation and enrichment, and ameliorates brain injury[J]. Scientific reports, 2016, 6.

16. 16.Wang R Y, Lin X J, Yang G Y, et al. Effect of hirulog-like peptide on middle cerebral artery occlusion-induced brain injury in mice[J]. Neuroscience, 2014, 277: 568-576.

17. 17.Wang Y, Zhang Y, Huang J, et al. Increase of circulating miR-223 and insulin-like growth factor-1 is associated with the pathogenesis of acute ischemic stroke in patients[J]. BMC neurology, 2014, 14(1): 77.

18. 18.Li Q, Zhang T, Wang J, et al. Rapamycin attenuates mitochondrial dysfunction via activation of mitophagy in experimental ischemic stroke[J]. Biochemical and biophysical research communications, 2014, 444(2): 182-188.

19. 19.He X, Lu Y, Lin X, et al. Optical inhibition of striatal neurons promotes focal neurogenesis and neurobehavioral recovery in mice after middle cerebral artery occlusion[J]. Journal of Cerebral Blood Flow & Metabolism, 2016: 0271678X16642242.

20. 20.Miao P, Wu Z, Li M, et al. Synchrotron Radiation X-Ray Phase-Contrast Tomography Visualizes Microvasculature Changes in Mice Brains after Ischemic Injury[J]. Neural plasticity, 2016, 2016.

21. 21.Tang G, Liu Y, Zhang Z, et al. Mesenchymal stem cells maintain blood‐brain barrier integrity by inhibiting aquaporin‐4 upregulation after cerebral ischemia[J]. Stem Cells, 2014, 32(12): 3150-3162.

22. 22.Mu Z H, Jiang Z, Lin X J, et al. Vessel dilation attenuates endothelial dysfunction following middle cerebral artery occlusion in hyperglycemic rats[J]. CNS neuroscience & therapeutics, 2016.

23. 23.Wang Z, Song F, Li J, et al. PET demonstrates functional recovery after treatment by Danhong injection in a rat model of cerebral ischemic-reperfusion injury[J]. Evidence-Based Complementary and Alternative Medicine, 2014, 2014.

24. 24.Lin X, Miao P, Mu Z, et al. Development of functional in vivo imaging of cerebral lenticulostriate artery using novel synchrotron radiation angiography[J]. Physics in medicine and biology, 2015, 60(4): 1655.

25. 25.Miao P, Lin X, Feng S, et al. In-vivo brain blood flow imaging based on laser speckle contrast imaging and synchrotron radiation microangiography[J]. Laser Physics, 2014, 24(8): 085603.

26. 26.Lv B, Li F, Han J, et al. Hif-1α overexpression improves transplanted bone mesenchymal stem cells survival in rat MCAO stroke model[J]. Frontiers in Molecular Neuroscience, 2017, 10.

27. 27.Liu Y, Tang G H, Sun Y H, et al. The protective role of Tongxinluo on blood–brain barrier after ischemia–reperfusion brain injury[J]. Journal of Ethnopharmacology, 2013, 148(2): 632-639.

28. 28.Tang Y, Cai B, Yuan F, et al. Melatonin pretreatment improves the survival and function of transplanted mesenchymal stem cells after focal cerebral ischemia[J]. Cell transplantation, 2014, 23(10): 1279-1291.

29. 29.Li Q, Tang G, Xue S, et al. Silica-coated superparamagnetic iron oxide nanoparticles targeting of EPCs in ischemic brain injury[J]. Biomaterials, 2013, 34(21): 4982-4992.

30. 30Tang Y, Cai B, Yuan F, et al. CT-0998 Accepted 04/19/2013 for publication in “Cell Transplantation” Melatonin pretreatment improves the survival and function of transplanted mesenchymal stem cells after focal cerebral ischemia[J]. 2013.

31. 31.Yue X H, Tong J Q, Wang Z J, et al. Steroid sulfatase inhibitor DU-14 protects spatial memory and synaptic plasticity from disruption by amyloid β protein in male rats[J]. Hormones and behavior, 2016, 83: 83-92.

32. 32.Yao C, Zhang J, Liu G, et al. Neuroprotection by (-)-epigallocatechin-3-gallate in a rat model of stroke is mediated through inhibition of endoplasmic reticulum stress[J]. Molecular medicine reports, 2014, 9(1): 69-72.

33. 33.Falei Yuan, Yaohui Tang, Xiaojie Lin, Yan Xi, Yongjing Guan, Tiqiao Xiao, Jun Chen, Zhijun Zhang, Guo-Yuan Yang, and Yongting Wang.Optimizing Suture Middle Cerebral Artery Occlusion Model in C57BL/6 Mice Circumvents Posterior Communicating Artery Dysplasia. Journal of Neurotrauma. May 1, 2012, 29(7): 1499-1505. 

34. 34.Nathalie Cloutier, Alexandre Paré, Richard W. Farndale, H. Ralph Schumacher, Peter A. Nigrovic, Steve Lacroix, and Eric Boilard. Platelets can enhance vascular permeability. Blood August 9, 2012, vol. 120 no. 6 1334-1343. 

35. 35.Jun Cai, Chuan He, Falei Yuan, Lujia Chen, Feng Ling. A novel haemodynamic cerebral aneurysm model of rats with normal blood pressure. Journal of Clinical Neuroscience, January 2012, Volume 19, Issue 1, Pages 135–138. 

36. 36.Bohua Xie, Peng Miao, Yuhao Sun, Yongting Wang, Guo-Yuan Yang. Micro-Computed Tomography for Hemorrhage Disruption of Mouse Brain Vasculature. Translational Stroke Research, July 2012, Volume 3, Issue 1 Supplement, pp 174-179. 

37. 37.Rongbiao Tang, Yan Xi, Wei-Min Chai, Yongting Wang, Yongjing Guan, Guo-Yuan Yang, Honglan Xie and Ke-Min Chen. Microbubble-based synchrotron radiation phase contrast imaging: basic study and angiography applications. Phys. Med. Biol., 2011, Vol 56:  3503.

38. 38.Mingfeng Xia, Minjuan Bian, Qian Yu, Jie Liu, Yufang Huang, Xueting Jin, Shiduo Lu, Mei Yu and Fang Huang. Cold water stress attenuates dopaminergic neurotoxicity induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in mice. Acta Biochim Biophys Sin, 2011,43 (6): 448-454.

39. 39.Cai J, Sun Y, Yuan F, Chen L, He C, Bao Y, Chen Z, Lou M, Xia W, Yang G and Ling F. A Novel Intravital Method to Evaluate Cerebral Vasospasm in Rat Models of Subarachnoid Hemorrhage: A Study with Synchrotron Radiation Angiography. PLoS ONE,2012, 7(3): e33366.

40. 40.Bin Cai, Yi Lin, Xie-Hua Xue, Ling Fang, Ning Wang, Zhi-Ying Wu. TAT-mediated delivery of neuroglobin protects against focal cerebral ischemia in mice. Experimental Neurology, January 2011, Volume 227, Issue 1, Pages 224–23。