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DNA-In® Stem Transfection Reagent

Optimized for maximum DNA delivery in Stem Cells 

The introduction of transgenes into stem cells has shown to be a valuable experiment technique for studying stem cell biology. Transfecting stem cells without inhibiting cell viability and cell growth has shown to be difficult. DNA-In® Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA into a wide range of stem cells, including neural stem cells. Formulated and optimized specifically for embryonic and adult stem cells, DNA-In® Stem is a new-generation transfection reagent that enables high efficiency transfection while maintaining maximum cell viability and cell growth. 

• Optimized formulation for DNA delivery into primary and iPS cells.

• Maximum transfection efficiency and cell viability.

• Requires only low amounts of DNA for maximum expression and exceptionally low toxicity.

• Robust, reproducible performance across a wide range of DNA concentrations.

Learn about High Efficiency CRISPR-Cas9 Delivery in Stem Cells withEditPro™ Stem

Learn also about the new DNA-In® CRISPR transfection reagent for primary cells

High efficiency, low cytotoxicity

Figure 1. Data above show cells transfected with DNA-In® Stem Transfection Reagent. Cells were plated in 24-well plates to give 60-70% confluency on the day of transfection. Cells were transfected with various amounts of a DNA plasmid, containing eGFP behind an EF1-alpha promoter using 1µl DNA-In® Stem. Cells were analyzed 24-hours and 48-hours (not shown) after transfection.  Above, representative images of normal human induced pluripotent stem cells  (iPSC)-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived mesenchymal stem cells (MSC) and Huntington's Disease (HD)-specific iPSCs show consistently high efficiency with low toxicity when using DNA-In® Stem transfection reagent.

Outperforms competitors across a range of cell types

Figure 2 (a-d). DNA-In® Stem requires up to 4X less DNA vs competitor reagents for maximum performance.  Human iPSC-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived Mesencymal Stem Cells and Huntington's Disease (HD)-specific iPSCs plated in 24-well plates were transfected with DNA-In® Stem, Lipofectamine®2000 (L2000) and 3000 (L3000) using various amounts (µl) of reagent.  Cells transfected with DNA-In® Stem were transfected with both low and high amounts of DNA. Cells were also transfected with L2000 and L3000 using their recommended 500ng of DNA.

Consistent, robust performance

Figure 3 (a-d). DNA-In® Stem shows robust performance across a range of DNA amounts in stem cells to product high transfection efficiency. Stem cells, including plated in 24-well plates were transfected with various amounts of DNA-In® Stem and DNA. Cells were then observed 24-hours (above data) and 48-hours (not shown) post-transfection. Above data for Human iPSC-derived neural stem cells (NSC), Human embryonic stem cells, adipose-derived Mesencymal Stem Cells and Huntington's Disease (HD)-specific iPSCs show consistently high efficiency with low toxicity when using DNA-In® Stem Transfection Reagent.