DNA-In® A549 Transfection Reagent

A Transfection Reagent for A549 Cells 

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines.  Features

 Optimized for maximum transfection efficiency and cell viability in A549 cells.

• One-step process: simply add DNA-In® A549 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells

• Minimal toxicity - no need to replace medium after transfection

 Maximum Transfection Efficiency in A549

Above, A549 cells were plated at 70% confluency and were transfected  with the EF-1 alpha promoter-GFP 24 hours after plating. DNA-In® A549 transfected cells (left image) show significantly higher expression vs LF3K transfected cells (right image).

Superior Transfection Efficiency and Maximum Cell Viability

During its development and validation process, DNA-In® A549 Transfection Reagent underwent extensive testing in many primary and cultured cells.  In a head-to-head comparison study, DNA-In A549 Reagent  consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents. 

The following charts show side-by-side comparisons of DNA-In® A549, Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in A549 cells:

Simple, Quick-Start Protocol