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454 GS Junior系統(tǒng)將增加測序讀長及推出新自動化方案

瀏覽次數(shù)：3266　發(fā)布日期：2012-5-14　 來源：本站　本站原創(chuàng)，轉(zhuǎn)載請注明出處
 
454 GS Junior測序儀最近推出了其Phase B的 一系列改進，包括新版本的測序軟件v2.7，相比以前v2.5軟件，對于圖像處理有明顯提升，可以帶來更加穩(wěn)定的測序結(jié)果，尤其是擴增子的測序。同時，454公司也將在2012年-2013年間陸續(xù)推出一系列提高測序性能以及自動化工作流程的GS Junior升級方案。GS Junior系統(tǒng)在傳染病，癌癥，環(huán)境微生物及農(nóng)業(yè)研究方面都有廣泛應(yīng)用。1

新的測序軟件v2.7提高了測序穩(wěn)定性以及測序效率，除了在圖像處理方面的提升外也在GS De Novo Assembler, GS Reference Mapper以及GS Amplicon Variant Analyzer軟件上增加了一些新的功能。在實驗方面，paired end測序（可以獲得跨越3 kb, 8 kb或者20 kb區(qū)域的兩段序列）推出了使用GS FLX Titanium Rapid Library Preparation Kits的文庫制備方案，加快建庫速度，同時可以為樣本添加特異性標(biāo)簽進行混合測序。
 
此次特別宣布了關(guān)于GS Junior系統(tǒng)的一系列后續(xù)升級方案，包括增加測序讀長，自動化文庫制備和emPCR工作流程的計劃。這些方面的提升將幫助原先使用傳統(tǒng)Sanger測序方法的研究人員方便轉(zhuǎn)移到454測序平臺上，無論是基礎(chǔ)研究還是轉(zhuǎn)化醫(yī)學(xué)領(lǐng)域的研究人員，能使用GS Junior系統(tǒng)。這些改進預(yù)計最快在今年的晚些時候或者明年年初正式推出。
 
454 Life Sciences總裁Thomas Schinecker先生說道：“我們很高興看到GS Junior系統(tǒng)在全球得到推廣，同時也很興奮地向大家宣布我們?yōu)椴粩嗵岣咂湫阅�、簡便操作所做的各種努力及規(guī)劃。這些改進將使得454的長度長平臺更加人性化，使得更多傳統(tǒng)測序使用者可以轉(zhuǎn)移到二代測序平臺上�！�
 
1 了解更多GS Junior相關(guān)文獻(xiàn)，請瀏覽  http:///publications. 
 
 
 
Roche Launches Software Upgrade for GS Junior System, Announces Long Read Improvements and Workflow Automation for 2012/2013
Apr 16, 2012 - fuchsc8
 
Roche announced today the immediate availability of a new software upgrade and further upcoming sequencing performance and workflow improvements to the company’s 454 GS Junior System. Now a widely published benchtop next-generation sequencing platform, the GS Junior System is rapidly advancing research worldwide in areas of infectious disease, cancer, environmental microbiology and agriculture1.
 
The new software package (v2.7) improves sequencing consistency and robustness and also includes functionality improvements to the GS De NovoAssembler, GS Reference Mapper, and GS Amplicon Variant Analyzer Software. The company also announced the immediate availability of new protocols for paired end sequencing (3 kb, 8 kb, 20 kb span) using the GS FLX Titanium Rapid Library Preparation Kits. The protocols enable simple sample multiplexing for paired end projects and reduce overall prep time.
 
In addition, the company has announced upcoming improvements to the GS Junior System, which include extension to sequencing read lengths and automation of the library preparation and emPCR workflow. The improvements will further ease the transition of projects from traditional Sanger capillary sequencing to 454 Sequencing Systems in basic and translational research laboratories around the world. The planned improvements will first become available in late 2012 and during 2013.
 
 “We are pleased by the adoption of the GS Junior System worldwide and are excited to announce our efforts to continuously improve performance and ease of use of the platform,” said Thomas Schinecker, President of 454 Life Sciences, a Roche Company. “These improvements make our long read sequencing solution more user friendly and enable customers to convert projects from Sanger to next-generation sequencing more easily.”

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