一、細(xì)胞破碎應(yīng)用
Cell Rupture, Disruption, Lysis are methods for disrupting cell walls and cell membranes to release biological molecules, whilst maintaining intracellular contents integrity.
細(xì)胞破裂、破碎、溶解是細(xì)胞壁和細(xì)胞膜破碎以釋放生物分子,同時保留細(xì)胞內(nèi)含物完整的方法。
Cell Disruption / Lysis細(xì)胞破碎/裂解
The apparatus used for cell disruption is often referred to as a 'French pressure cell press', or 'French Press'. Our 'Stansted' range of 'Benchtop' Cell Disruptors provide an
industry-leading, powerful and extremely user friendly replacement for the French ® pressure cell system with capabilities for batch and continuous operation.
用于細(xì)胞破碎的儀器常會被聯(lián)想到'French pressure cell press'或者 'French Press'。我們的“'Stansted' 系列 'Benchtop'細(xì)胞破碎儀提供業(yè)界領(lǐng)先、強(qiáng)大和極為用戶友好的French ®類型高壓細(xì)胞系統(tǒng),具有單批次和連續(xù)運(yùn)行的功能。
Our very high pressure performance up to 58,000 psi (4,000 bar) ensure our systems can handle the toughest cells while a versatile range of flexible lysing valves ensure finely controlled operation even when working the softest.
我們非常高壓力性能達(dá)58,000 psi (4000bar)保證我們的系統(tǒng)可以處理最堅硬的細(xì)胞,同時多功能范圍的靈活的溶解閥保證非常好的控制處理最柔軟的樣品。
Common Applications include共同的應(yīng)用包括:
E.coli大腸桿菌
Yeasts (s cerivises, Pichia)酵母菌
Algae藻類
Mamalllian cells馬馬利亞血細(xì)胞
Bacillus 桿菌
Staph a.葡萄桿菌
CBD / Cannabinoids大麻素類
... and many others
Enzyme preparation, Cell extract preparation Bacterial and plant tissue disintegration Recovery of intercellular proteins and nucleic acid. Preparation of viral antigens, membrane
preparation for phizobia and pseudomonads ...... etc.)
生物細(xì)胞破壁: (大腸桿菌、酵母菌、霉菌......等等) 酶的分離和隔離,細(xì)菌和植物組織斷裂,細(xì)胞內(nèi)蛋白質(zhì)和核酸的回收,制備EIA測試包的病毒性抗原,phizobia和假單體的膜制備,胚芽和毒素的回收,蛋白質(zhì)純化,細(xì)胞提取制備等 范圍包括:微生物(大腸桿菌、酵母菌、酶菌)、藻類、動物細(xì)胞和組織、植物細(xì)胞和組織、昆蟲、病毒、環(huán)境樣品等。
Our scalable technology offer practical processing from 1ml, through bench top, pilot and production systems with flow rate in excess of 1000 l/hr.
我們的可擴(kuò)展技術(shù)提供實(shí)際處理從1ml,實(shí)驗(yàn)桌面型,中試和生產(chǎn)系統(tǒng)流量超過1000升每小時。
二、生物技術(shù):細(xì)胞破碎應(yīng)用例子
生物細(xì)胞破壁:
(大腸桿菌、酵母菌、霉菌......等等)
酶的分離和隔離,細(xì)菌和植物組織斷裂,細(xì)胞內(nèi)蛋白質(zhì)和核酸的回收,制備EIA測試包的病毒性抗原,phizobia和假單體的膜制備,胚芽和毒素的回收,蛋白質(zhì)純化,細(xì)胞提取制備等
范圍包括:微生物(大腸桿菌、酵母菌、酶菌)、藻類、動物細(xì)胞和組織、植物細(xì)胞和組織、昆蟲、病毒、環(huán)境樣品等。
1、大腸桿菌破碎前后鏡檢圖。
1)未破碎
2)100MPa破碎一遍
3)100MPa破碎二遍
4)100MPa破碎三遍
5)100MPa破碎四遍
2、Lysis of
Pseudomonas aeruginosa
Non-lysed Sample
Lysed Sample
10ml of cells run once through machine at ~100 MPa
3、酵母菌破碎效果鏡檢圖
三、各類細(xì)胞、細(xì)菌建議使用的壓力
動物細(xì)胞(淋巴細(xì)胞) 1 to 15 MPa
細(xì)胞器與線粒體 1 to 15 MPa
寄生物(錐體蟲) 1 to 15 MPa
Sperm 1 to 35 MPa
線蟲類 1 to 35 MPa
大腸桿菌 30 to 140 MPa
動物組織 70 to 150 MPa
植物組織與藻類(藍(lán)細(xì)菌)35 to 150 MPa
酵母菌 100 to 250 MPa
角質(zhì)層 140 to 200 MPa
DNA 100 to 250 MPa
硬細(xì)菌(葡萄球菌、鏈球菌) 150 to 320 MPa
霉菌 150 to 320 MPa