利用Dualex便攜式葉綠素儀測量作物葉片葉綠素濃度的研究

瀏覽次數(shù)：1242　發(fā)布日期：2022-4-27　來源：本站　僅供參考，謝絕轉(zhuǎn)載，否則責(zé)任自負(fù)

Dualex是一款源自于法國國家科學(xué)院 (CNRS)及巴黎第十一大學(xué)技術(shù)，由奧地利PESSL公司生產(chǎn)（原法國Force-A公司）開發(fā)的新型多功能葉片測量儀。它可同時準(zhǔn)確測量葉片的葉綠素含量、葉片表層的類黃酮和花青素含量，適用于植物生理學(xué)和農(nóng)學(xué)（如水稻葉綠素濃度，玉米氮素狀況，葡萄藤等）相關(guān)研究。

其測量對象可以是單子葉植物，雙子葉植物或多年生植物。這款設(shè)備簡單易用，可進(jìn)行實時和非破壞性測量。由于不需要校準(zhǔn)標(biāo)定和事先的樣品制備，測量工作可在實驗室或現(xiàn)場完成。此外，該設(shè)備在各種溫度的和環(huán)境光照條件下均可正常使用。系統(tǒng)采用專利設(shè)計，光學(xué)傳感器可進(jìn)行簡單、快速、無損測量葉片中的葉綠素、多酚以及花青素。

Dualex便攜式智能氣象-葉綠素-花青素-黃酮醇測量儀

摘要

使用便攜式葉綠素儀（PCM）在田間準(zhǔn)確測量葉片葉綠素濃度（LChl）對于支持利用遙感繪制作物氮素狀況時空變化圖的方法開發(fā)至關(guān)重要。已經(jīng)開發(fā)了幾種PCM，用于在現(xiàn)場瞬時和非破壞性地測量LChl，但是，它們的讀數(shù)是相對量，需要使用轉(zhuǎn)換函數(shù)將其轉(zhuǎn)換為實際LChl值。本研究的目的是調(diào)查實際LChl與三種PCM（SPAD-502、CCM-200和Dualex-4）獲得的PCM讀數(shù)之間的關(guān)系。2016年，在四種作物上在加拿大安大略省渥太華的加拿大農(nóng)業(yè)和農(nóng)業(yè)食品中心試驗農(nóng)場進(jìn)行了田間試驗：玉米（Zea mays L.）、大豆（Glycine max L.Merr.）、玉米和大豆，春小麥（小麥屬）和油菜（甘藍(lán)型油菜屬）。為了評估其他因素（葉片內(nèi)部結(jié)構(gòu)、除葉綠素以外的葉片色素以及LChl分布的異質(zhì)性）對轉(zhuǎn)換函數(shù)的影響，使用PROSPECT-D模型進(jìn)行了全局敏感性分析，以模擬不同條件下的PCM讀數(shù)。結(jié)果表明，與SPAD-502和CCM-200相比，Dualex-4在實際LChl測量中具有更好的性能，對所有四種測試作物使用通用轉(zhuǎn)換函數(shù)。對于SPAD-502和CCM-200，讀數(shù)中的誤差隨著LChl的增加而增加。靈敏度分析表明，與葉結(jié)構(gòu)相比，非均勻LChl分布更容易導(dǎo)致校準(zhǔn)函數(shù)的偏差。Dualex-4的讀數(shù)比其他兩個PCM的讀數(shù)更能限制這些影響。

關(guān)鍵詞：葉片葉綠素濃度；便攜式葉綠素儀；作物；PROSPECT-D；敏感性分析；遙感；輻射傳輸模型

Assessment of Portable Chlorophyll Meters for Measuring Crop Leaf Chlorophyll Concentration

Abstract

Accurate measurement of leaf chlorophyll concentration (LChl) in the field using a portable chlorophyll meter (PCM) is crucial to support methodology development for mapping the spatiotemporal variability of crop nitrogen status using remote sensing. Several PCMs have been developed to measure LChl instantaneously and non-destructively in the field, however, their readings are relative quantities that need to be converted into actual LChl values using conversion functions. The aim of this study was to investigate the relationship between actual LChl and PCM readings obtained by three PCMs: SPAD-502, CCM-200, and Dualex-4. Field experiments were conducted in 2016 on four crops: corn (Zea mays L.), soybean (Glycine max L. Merr.), spring wheat (Triticum aestivum L.), and canola (Brassica napus L.), at the Central Experimental Farm of Agriculture and Agri-Food Canada in Ottawa, Ontario, Canada. To evaluate the impact of other factors (leaf internal structure, leaf pigments other than chlorophyll, and the heterogeneity of LChl distribution) on the conversion function, a global sensitivity analysis was conducted using the PROSPECT-D model to simulate PCM readings under different conditions. Results showed that Dualex-4 had a better performance for actual LChl measurement than SPAD-502 and CCM-200, using a general conversion function for all four crops tested. For SPAD-502 and CCM-200, the error in the readings increases with increasing LChl. The sensitivity analysis reveals that deviations from the calibration functions are more induced by non-uniform LChl distribution than leaf architectures. The readings of Dualex-4 can have a better ability to restrict these influences than those of the other two PCMs.

Keywords: leaf chlorophyll concentration; portable chlorophyll meter; crop; PROSPECT-D; sensitivity analysis; remote sensing; radiative transfer model

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