The Virus Platform provided packaging services for a variety of viral types. The packaged viruses are classified two categories: DNA integrative viruses and DNA non-integrative viruses. DNA integrative viruses refer to retrovirus and classical lentivirus whereas DNA non-integrative viruses refer to modified lentivirus, baculovirus, adenovirus, adeno-associated virus and baculovirus. The viral titer and purity are guaranteed for effective infection for both cultured cells and tissues.

1. Classical Lentivirus and retrovirus

Lentivirus is a genus of slow viruses of the Retroviridae family, characterized by a long incubation period. Lentiviruses and retroviruses can deliver a significant amount of genetic information into the DNA of the host cell and have the unique ability among retroviruses of being able to replicate in non-dividing cells, so they are one of the most efficient methods of a gene delivery vector. Like many other types of retroviruses, lentivirus and retrovirus are excellent research tools for stable introduction of a gene product into in vitro systems or animal models for stable expression. Another common application is to use a lentivirus or retrovirus to introduce  an ShRNA cassette (e.g., ShRNA) for stable gene silencing. The titer of lentiviruses and retroviruses ranges from 1x10⁶ to 1x10⁸ TU/ml.

2. Modified Lentivirus

To develop efficient transient transfection methods, Biowit has also established a modified lentiviral vector system by inactivating the integrative elements of classical lentivruses. The modified lentivirus can efficiently infect a variety of mammalian cells like classical lentivirus whereas its viral DNA does not integrate into the host genome, thus it serve as an excellent alternative for routine transient transfection while it can also avoid the integrative side effects of classical lentivirus. The titer of non-integrative lentiviruses ranges from 1x10⁶ to 1x10⁸ TU/ml.

3. Adenovirus

Adenoviruses are medium-sized (90–100 nm), nonenveloped icosahedral viruses composed of a nucleocapsid and a double-stranded linear DNA genome. It can infect various species of vertebrates. Adenoviruses possess a linear dsDNA genome and are able to replicate in the nucleus of mammalian cells using the host’s replication machinery. Adenovirus is used as a vehicle to administer targeted therapy, in the form of recombinant DNA or protein. The titer of packaged adenoviruses ranges from 1x10¹⁰-1x 10¹² pfu/ml.

4. Adeno-associated virus

Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease. Moreover, virus infection causes a very mild immune response. AAV can infect both dividing and non-dividing cells These features make AAV a very attractive candidate for creating viral vectors for gene therapy, and for the creation of isogenic human disease models. Recent human clinical trials using AAV for gene therapy in the retina have shown promise. Different AAV serotypes have demonstrated patterns for the target genes in terms of expression time and host selectivity.  The Biowit virus platform provides a variety of AAV2 serotypes including AAV2/1, AAV2/2, AAV2/3, AAV2/4, AAV2/5, AAV2/6, AAV2/7, AAV2/8 and AAV2/9. The titer of packaged AAV  ranges from 1x10¹¹-1x 10¹² vg/ml.

Biowit Technologies Ltd. is a high-tech company specialized in providing R&D services and related products in the biological field. As a technology-driven and customer-focused company, BioWit provides a broad and integrated service and product portfolio including molecular biology, virus packaging (e.g., adenovirus, AAV, lentivirus, retrovirus and baculovirus), protein expression, cell biology, stem cells and transgenic models. One of the milestones achieved by Biowit is its recent sucess in the development of a series of serum-containing media as well as serum-free  or animal -free media for stem cell culture, which are tailored for clinical applications. The core technology team members have engaged in research and development for many years in world-famous academic institutions or prestigious drug companies. Biowit has established collaboration with international prestigious research institutions. The worldwide collaboration enables Biowit to timely renew its biotechnologies (http://www.biowit.com)

    百恩維生物科技有限公司（BioWit Technologies），是一家專業(yè)從事生物技術(shù)研發(fā)并提供相關(guān)技術(shù)服務(wù)和產(chǎn)品的高新技術(shù)企業(yè)。公司目前提供的技術(shù)服務(wù)和產(chǎn)品主要包括分子生物學(xué)技術(shù)服務(wù)、腺病毒（Adenovirus)、慢病毒(Lentivirus)、腺相關(guān)病毒（AAV）、桿狀病毒（Baculovirus)等包裝技術(shù)服務(wù)、蛋白質(zhì)表達(dá)與純化技術(shù)服務(wù)（包括原核細(xì)菌、桿狀病毒-昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞等）、高效轉(zhuǎn)染試劑（HET Kit)、細(xì)胞因子(如hLif、mLif、aFGF、bFGF、EGF、TGFα、TGFβ等）、干細(xì)胞培養(yǎng)基(如胎胚干細(xì)胞培養(yǎng)基、間充質(zhì)干細(xì)胞培養(yǎng)基等）、干細(xì)胞的分離和存儲以及個(gè)體特異性誘導(dǎo)型多能干細(xì)胞的制備和存儲, 其中干細(xì)胞無血清或無動(dòng)物源培養(yǎng)是專門為臨床打造和設(shè)計(jì)的快速擴(kuò)增細(xì)胞的培養(yǎng)基同時(shí)又能很好地維持其干細(xì)胞的潛能。公司的長期目標(biāo)是推出人類重大疑難疾病的干細(xì)胞治療及基因治療臨床方案和預(yù)防措施，重點(diǎn)研究神經(jīng)疾病、視網(wǎng)膜疾病、白血病、血友病、艾滋病和肝炎的干細(xì)胞治療和基因治療方案，及提供高效的預(yù)防動(dòng)物或人的傳染病、流行病的疫苗(http://www.biowit.com)。