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電泳時的DNA分子量Marker和Agarose濃度的選擇

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電泳時的DNA分子量Marker和Agarose濃度的選擇

DNA片段長度

Agarose濃度 

使用Marker種類

200 bp以下

3%以上

 фX174-Hae digest DNA Marker
 фX174-Hinc digest DNA Marker
 DL500™DNA Marker
 DL1,000™DNA Marker
 DL2,000™DNA Marker
 20 bp DNA Ladder Marker
 50 bp DNA Ladder Marker
 100 bp DNA Ladder Marker
 150 bp DNA Ladder Marker
 Wide Range DNA Marker (100~6,000)

200 bp~700 bp

2%~3%

 фX174-Hae digest DNA Marker
 фX174-Hinc digest DNA Marker
 DL500™ DNA Marker
 DL1,000™ DNA Marker
 DL2,000™ DNA Marker
 DL5,000™ DNA Marker
 50 bp DNA Ladder Marker
 100 bp DNA Ladder Marker
 150 bp DNA Ladder Marker
 200 bp DNA Ladder Marker
 250 bp DNA Ladder Marker
 500 bp DNA Ladder Marker
 Wide Range DNA Marker (100~6,000)

700 bp~1,500 bp

1%~2%

 λ-EcoT14digest DNA Marker
 фX174-Hae digest DNA Marker
 фX174-Hinc digest DNA Marker
 DL2,000™ DNA Marker
 DL5,000™ DNA Marker
 DL10,000™ DNA Marker
 50 bp DNA Ladder Marker
 100 bp DNA Ladder Marker
 150 bp DNA Ladder Marker
 200 bp DNA Ladder Marker
 250 bp DNA Ladder Marker
 500 bp DNA Ladder Marker
 Wide Range DNA Marker (100~6,000)
 Wide Range DNA Marker (500~12,000)
 Wide Range DNA Marker (500~15,000)

1,500 bp~5,000 bp

0.7%~1%

 λ-EcoT14digest DNA Marker
 λ-Hind digest DNA Marker
 DL5,000™ DNA Marker
 DL10,000™ DNA Marker
 DL15,000™ DNA Marker
 Supercoiled DNA Ladder Marker
 200 bp DNA Ladder Marker
 250 bp DNA Ladder Marker
 500 bp DNA Ladder Marker
 Wide Range DNA Marker (100~6,000)
 Wide Range DNA Marker (500~12,000)
 Wide Range DNA Marker (500~15,000)
 1 Kbp DNA Ladder Marker

 5,000 bp以上

0.7%以下

 λ-EcoT14digest DNA Marker
 λ-Hind digest DNA Marker
 DL10,000™ DNA Marker
 DL15,000™ DNA Marker
 Supercoiled DNA Ladder Marker
 Wide Range DNA Marker (500~12,000)
 Wide Range DNA Marker (500~15,000)
 1 Kbp DNA Ladder Marker


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