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                                電泳時的DNA分子量Marker和Agarose濃度的選擇

                                瀏覽次數(shù):10568 發(fā)布日期:2009-1-21  來源:本站 僅供參考,謝絕轉(zhuǎn)載,否則責(zé)任自負

                                電泳時的DNA分子量Marker和Agarose濃度的選擇

                                DNA片段長度

                                Agarose濃度 

                                使用Marker種類

                                200 bp以下

                                3%以上

                                 фX174-Hae digest DNA Marker
                                 фX174-Hinc digest DNA Marker
                                 DL500™DNA Marker
                                 DL1,000™DNA Marker
                                 DL2,000™DNA Marker
                                 20 bp DNA Ladder Marker
                                 50 bp DNA Ladder Marker
                                 100 bp DNA Ladder Marker
                                 150 bp DNA Ladder Marker
                                 Wide Range DNA Marker (100~6,000)

                                200 bp~700 bp

                                2%~3%

                                 фX174-Hae digest DNA Marker
                                 фX174-Hinc digest DNA Marker
                                 DL500™ DNA Marker
                                 DL1,000™ DNA Marker
                                 DL2,000™ DNA Marker
                                 DL5,000™ DNA Marker
                                 50 bp DNA Ladder Marker
                                 100 bp DNA Ladder Marker
                                 150 bp DNA Ladder Marker
                                 200 bp DNA Ladder Marker
                                 250 bp DNA Ladder Marker
                                 500 bp DNA Ladder Marker
                                 Wide Range DNA Marker (100~6,000)

                                700 bp~1,500 bp

                                1%~2%

                                 λ-EcoT14digest DNA Marker
                                 фX174-Hae digest DNA Marker
                                 фX174-Hinc digest DNA Marker
                                 DL2,000™ DNA Marker
                                 DL5,000™ DNA Marker
                                 DL10,000™ DNA Marker
                                 50 bp DNA Ladder Marker
                                 100 bp DNA Ladder Marker
                                 150 bp DNA Ladder Marker
                                 200 bp DNA Ladder Marker
                                 250 bp DNA Ladder Marker
                                 500 bp DNA Ladder Marker
                                 Wide Range DNA Marker (100~6,000)
                                 Wide Range DNA Marker (500~12,000)
                                 Wide Range DNA Marker (500~15,000)

                                1,500 bp~5,000 bp

                                0.7%~1%

                                 λ-EcoT14digest DNA Marker
                                 λ-Hind digest DNA Marker
                                 DL5,000™ DNA Marker
                                 DL10,000™ DNA Marker
                                 DL15,000™ DNA Marker
                                 Supercoiled DNA Ladder Marker
                                 200 bp DNA Ladder Marker
                                 250 bp DNA Ladder Marker
                                 500 bp DNA Ladder Marker
                                 Wide Range DNA Marker (100~6,000)
                                 Wide Range DNA Marker (500~12,000)
                                 Wide Range DNA Marker (500~15,000)
                                 1 Kbp DNA Ladder Marker

                                 5,000 bp以上

                                0.7%以下

                                 λ-EcoT14digest DNA Marker
                                 λ-Hind digest DNA Marker
                                 DL10,000™ DNA Marker
                                 DL15,000™ DNA Marker
                                 Supercoiled DNA Ladder Marker
                                 Wide Range DNA Marker (500~12,000)
                                 Wide Range DNA Marker (500~15,000)
                                 1 Kbp DNA Ladder Marker


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