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                                                              當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 細(xì)胞生物學(xué)試劑 > IL-15細(xì)胞因子
                                                              IL-15細(xì)胞因子
                                                              英文名稱:總訪問:104
                                                              國產(chǎn)/進(jìn)口:國產(chǎn)半年訪問:2
                                                              產(chǎn)地/品牌:廈門/逸漠IMMOCELL產(chǎn)品類別:細(xì)胞生物學(xué)試劑
                                                              規(guī)       格:10ug/瓶 最后更新:2024-2-20
                                                              貨       號(hào):IMF-I15
                                                              CAS   號(hào):
                                                              參考報(bào)價(jià):1200
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                                                              ▍Backgound

                                                              Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97% and 73% sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK)

                                                              cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain.

                                                              ▍Source

                                                              Recombinant Human IL-15 is expressed from Escherichia coli. It contains AA Asn 49 - Ser 162. Predicted molecular mass 15.5 kDa. This protein carries a polyhistidine tag at the N-terminus.

                                                              ecombinant Human IL-15 is expressed from Escherichia coli

                                                              ▍Endotoxin

                                                              Less than 1.0 EU per μg by the LAL method.

                                                              ▍AA Sequence

                                                              NWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS

                                                              ▍Purity

                                                              Purity >90% as determined by SDS-PAGE.

                                                              ▍Formulation

                                                              Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

                                                              ▍Reconstitution

                                                              Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

                                                              ▍Storage

                                                              For long term storage, the product should be stored at lyophilized state at -20°C or lower.

                                                              Please avoid repeated freeze-thaw cycles.

                                                              This product is stable after storage at:

                                                              -20°C to -70°C for 12 months in lyophilized state;

                                                              -70°C for 3 months under sterile conditions after reconstitution.

                                                              ▍SDS-PAGE

                                                              Human IL-15, His Tag on SDS-PAGE under reducing (R) condition

                                                              Human IL-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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