Description:

Rabbit polyclonal antibody

  Background:

Arabidopsis V-ATPase has three B subunits (AtVAB1, AtVAB2, and AtVAB3), which share 97.27% sequence identity and have two potential actin-binding sites, indicating that these AtVABs may have crucial functions in actin cytoskeleton remodeling and plant cell development.

  Synonyms:

VAB1/2/3, ATVAB1/2/3, V-ATPASE B SUBUNIT 1/2/3

  Immunogen:

KLH-conjugated synthetic peptide of VAB1/2/3 derived from Arabidopsis thaliana AT1G76030, AT4G38510, AT1G20260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

54 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Hordeum vulgare, Gossypium raimondii, Oryza sativa Japonica Group, Zea mays, Vitis vinifera, Sorghum bicolor, Populus trichocarpa, Medicago truncatula, Glycine max, Brassica rapa, Setaria viridis, Cucumis sativus, Brassica napus, Triticum aestivum, Spinacia oleracea, and 80-99% homologues with the sequence in Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.