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Catalogue Number | CYT-258 |
Synonyms | IGFBP-6, IBP-6, IGF-binding protein 6. |
Introduction | IGFBP6 plays a role in lipoprotein assembly and dietary cholesterol absorption. in addition to its acyltransferase activity, it may act as a ligase. may provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa. |
Description | IGFBP6 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 148-240 and having a molecular mass of 20 kDa including 4kDa His tag. IGFBP6 is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | IBP-6 was lyophilized from a concentrated (1mg/ml) solution containing 1X PBS, 0.1% SDS and 1mM DTT. |
Solubility | It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized IBP6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 80.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Applications | 1. Positive control for Western Blot. 2. Antibody production. 3. Protein Assay. |
Usage | ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |