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TNF a Human
英文名稱:TNF a Human總訪問:253
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規(guī)       格:CYT-223 最后更新:2024-12-5
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Catalogue Number CYT-223
Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Introduction Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Description Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Dalton.
The TNF-alpha is purified by standard chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.
Solubility It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Arg-Ser-Ser.
Biological Activity The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20MIU/mg.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.
References

1.Title: Radiation-triggered Tumor Necrosis Factor (TNF) ?-NF?B Cross-signaling Favors Survival Advantage in Human Neuroblastoma Cells*
Publication:First Published on April 28, 2011, doi: 10.1074/jbc.M110.193755 June 17, 2011 The Journal of Biological Chemistry, 286, 21588-21600.
Link:http://www.jbc.org/content/286/24/21588

2.Title:Bidirectional Concentration-Dependent Effects of Tumor Necrosis Factor Alpha in Shigella dysenteriae-Related Seizures.
Publication:INFECTION AND IMMUNITY, Apr. 2003, p. 2288–2291 Vol. 71, No. 40019-9567/03/$08.000 DOI: 10.1128/IAI.71.4.2288–2291.2003Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Link:http://iai.asm.org/content/71/4/2288.full.pdf

3.Title:Common Inhibitory Serine Sites Phosphorylated by IRS-1 Kinases, Triggered by Insulin and Inducers of Insulin Resistance.
Publication:First Published on April 19, 2007, doi: 10.1074/jbc.M610949200 June 22, 2007 The Journal of Biological Chemistry, 282, 18018-18027.
Link:http://www.jbc.org/content/282/25/18018.full

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