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Catalogue Number | CYT-350 |
Synonyms | IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor. |
Introduction | Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis. |
Description | Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton. The IL-6 is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | The protein was lyophilized without any additives. |
Solubility | It is recommended to reconstitute the lyophilized Interleukin 6 in 10mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions. |
Stability | Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser. |
Biological Activity | The ED50 as determined by the dose-dependant induction of 7TD1 cell proliferation, is 0.018 ng/ml corresponding to a Specific Activity of 55MIU/mg. |
References | Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice. Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 . Link:http://www.fasebj.org/content/24/8/2869.full |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |