| product information
| | background | | VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast. | | immunogen | | part of recombinant Arabidopsis thaliana VDR1 protein sequence Q9SJ13, At2g21860, excluding membrane spanning part | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | 50 µl | for reconstitution add 50 µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS12 2604 | anti-VDE1 | VIOLAXANTHIN DEEPOXIDASE 1 rabbit antibody
other antibodies to GreenCut proteins | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1 : 1000 with standard ECL (WB) |
| expected | apparent MW | | 59 | 52.86 kDa (minus a transit peptide) |
| confirmed reactivity | | Arabidopsis thaliana
|
| predicted reactivity | | Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera |
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | to be added when available |
| selected references | | to be added when available, antibody released in April 2013. |
application example
From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.
Courtesy of Dr. Rikard Fristedt, UCLA, USA.
bio-equip.com
北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時俱進地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標是把國外有特點的生物前沿產(chǎn)品引進中國，讓國內(nèi)科學研究機構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。
我們代理的產(chǎn)品覆蓋細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。
細胞生物學包含細胞培養(yǎng)，細胞遷移，細胞信號轉(zhuǎn)導等。
植物學包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。
分子生物學包含表觀遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。
免疫學包含抗體，各類檢測試劑盒等。
生物化學包含各類生化試劑，化學小分子，常規(guī)試劑等。
蛋白組學包含不同種屬的重組蛋白，天然提取蛋白，細胞因子等。
生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。
在公司全體員工的共同努力和國外生產(chǎn)商的支持下，在業(yè)務(wù)上實現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長春、石家莊、哈爾濱等重點城市建立了分支機構(gòu)或授權(quán)服務(wù)商，并以專業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強企業(yè)的中國區(qū)產(chǎn)品供應(yīng)商，以及中國科學院科研采購平臺的中標供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷售平臺，業(yè)務(wù)代表銷售平臺，針對科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡(luò)平臺和業(yè)務(wù)代表平臺的優(yōu)點，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨立的營銷團隊，分別配備不同的專業(yè)技術(shù)支持和客服人員，通過網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。
公司客戶遍布中國生命科學基礎(chǔ)研究、開發(fā)應(yīng)用、制藥、疾病診斷與控制、商品檢驗檢疫、生物技術(shù)公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領(lǐng)域。
我們追求目的是與研究機構(gòu)及合作伙伴之間誠信而專業(yè)的對話，力求建立長久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)，會使您實驗設(shè)計更有效，實驗操作更簡便，研究結(jié)果更可靠。
放眼未來，公司將努力實現(xiàn)更大的突破，將國外優(yōu)秀產(chǎn)品不斷引進國內(nèi)，同時積極推動國內(nèi)的產(chǎn)品銷往世界各地
