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application information | ||
recommended dilution | 1: 2000 with standard ECL (WB) | |
expected | apparent MW | 25-38 kDa for A. thaliana | |
confirmed reactivity | Arabidopsis thaliana, Digitaria sanguinalis, Echinochla crus-galli, Liquidambar formosana, N. tabacum thylakoid-bound APX, stromal APX; Panicum milaceum, Schima superba, Silene vulgaris, Spinacia oleracea (stromal APX, thylakoid-bound) | |
predicted reactivity | O.sativa probable L-ascorbate peroxidase 6 chloroplastic; S. lycopersicum thylakoid-bound APX; | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | ||
selected references | Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae) Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem. |
application example
5 to 20 μg of total leaf protein from Arabidopsis thaliana (left panel) and chloroplast fractions (thylakoids and soluble, right panel) was separated on 15% polyacrylamide gel with 6M urea and blotted on PVDF. Filters were blocked 1h with 5% BSA, incubated with anti-APX antibody (1: 2000, 1h) followed by incubation with secondary HRP-coupled anti rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL. AS08 368 is reactive to thylakoid (tAPX, 38 kDa), stromal (sAPX, 33 kDa), peroxisomal (pAPX, 31 kDa) and cytoplasmic (cAPX1 + cAPX2, 25 kDa) forms of ascorbate peroxidases. Copyright(C) 1998-2025 生物器材網(wǎng) 電話:021-64166852;13621656896 E-mail:info@bio-equip.com
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