當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

英文名稱：	Toc75 \| Protein TOC75-3, chloroplastic, POTRA domain 3	總訪問：	250
國(guó)產(chǎn)/進(jìn)口：	進(jìn)口	半年訪問：	9
產(chǎn)地/品牌：	agrisera	產(chǎn)品類別：	植物試劑
規(guī) 格：	AS08351	最后更新：	2024-12-5
貨號(hào)：
CAS 號(hào)：
參考報(bào)價(jià)：	詢價(jià)
立即詢價(jià) 電話咨詢

[發(fā)表評(píng)論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏]

分享：微信新浪微博

銷售商：北京啟維益成科技有限公司

查看該公司所有產(chǎn)品 >>

產(chǎn)品介紹
公司簡(jiǎn)介

product information

background

OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

immunogen

psTOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

antibody format

rabbit;

polyclonal;

serum;

lyophilized

quantity

200 µl

- for reconstitution add 200 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB), flow cytometry (FACS), immunolocalization (IL)

related products

AS08 345 | anti- Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 rabbit antibodies

additional information

to be added when available

application information
recommended dilution		1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
expected \| apparent MW		88 \| 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
confirmed reactivity		Arabidopsis thaliana, Nicotiana tabacum, Pisum sativum, Spinacia oleracea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
predicted reactivity		Ricinus communis, Vitis vinifera, Populus trichocarpa
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
selected references		to be added when available, antibody released February 2010

application example

580 ng of Chl of Pisum sativum plants (10 day old) (2, 4) and 10 µg of combined envelopes of Pisum sativum 10 day-old(1,3) were separated on 15% SDS-PAGE and blotted 2h to PVDF. PVDF was blocked 1h with 3% non-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 POTRA domain 3 antibodies AS08 351 (1:2000 and 1: 5000, 1h) and secondary donkey-anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 3% non fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with HRP substrate Peroxide solution & luminol detection reagent according to the manufacturers instructions (Millipore). Exposure time was 600 seconds.

Ashita Dave and Barry D. Bruce (UTK)

bio-equip.com

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗(yàn)人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長(zhǎng)期穩(wěn)定的合作關(guān)系，已成為眾多國(guó)際知名品牌的中國(guó)總代理或一級(jí)代理商；并且為滿足國(guó)內(nèi)客戶的最新需求，我們也與時(shí)俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國(guó)外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國(guó)，讓國(guó)內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。

我們代理的產(chǎn)品覆蓋細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。

細(xì)胞生物學(xué)包含細(xì)胞培養(yǎng)，細(xì)胞遷移，細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)等。

植物學(xué)包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。

分子生物學(xué)包含表觀遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。

免疫學(xué)包含抗體，各類檢測(cè)試劑盒等。

生物化學(xué)包含各類生化試劑，化學(xué)小分子，常規(guī)試劑等。

蛋白組學(xué)包含不同種屬的重組蛋白，天然提取蛋白，細(xì)胞因子等。

生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。

在公司全體員工的共同努力和國(guó)外生產(chǎn)商的支持下，在業(yè)務(wù)上實(shí)現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長(zhǎng)春、石家莊、哈爾濱等重點(diǎn)城市建立了分支機(jī)構(gòu)或授權(quán)服務(wù)商，并以專業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強(qiáng)企業(yè)的中國(guó)區(qū)產(chǎn)品供應(yīng)商，以及中國(guó)科學(xué)院科研采購(gòu)平臺(tái)的中標(biāo)供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進(jìn)了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷售平臺(tái)，業(yè)務(wù)代表銷售平臺(tái)，針對(duì)科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡(luò)平臺(tái)和業(yè)務(wù)代表平臺(tái)的優(yōu)點(diǎn)，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨(dú)立的營(yíng)銷團(tuán)隊(duì)，分別配備不同的專業(yè)技術(shù)支持和客服人員，通過網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。

公司客戶遍布中國(guó)生命科學(xué)基礎(chǔ)研究、開發(fā)應(yīng)用、制藥、疾病診斷與控制、商品檢驗(yàn)檢疫、生物技術(shù)公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領(lǐng)域。

我們追求目的是與研究機(jī)構(gòu)及合作伙伴之間誠(chéng)信而專業(yè)的對(duì)話，力求建立長(zhǎng)久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)，會(huì)使您實(shí)驗(yàn)設(shè)計(jì)更有效，實(shí)驗(yàn)操作更簡(jiǎn)便，研究結(jié)果更可靠。

放眼未來，公司將努力實(shí)現(xiàn)更大的突破，將國(guó)外優(yōu)秀產(chǎn)品不斷引進(jìn)國(guó)內(nèi)，同時(shí)積極推動(dòng)國(guó)內(nèi)的產(chǎn)品銷往世界各地

售后服務(wù)

相關(guān)視頻

資料下載

快速詢價(jià) 請(qǐng) 登錄或注冊(cè)在線詢價(jià)　(請(qǐng)留下您的聯(lián)系方式，以便供應(yīng)商聯(lián)系您)