product information background HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90. immunogen full length recombinant Hsp90-2 from Arabidopsis thaliana antibody format rabbit; polyclonal; serum; lyophilized quantity 100 µl - for reconstitution add100 µl of sterile water storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications Western blot (WB) related products collection of antibodies to plant HSP proteins additional information Antibody reacts more with constitutive isofrom Hsp90-2 than heat inducible Hsp90-1 - for a reference check with a western blot image below.

application information
recommended dilution		1: 3000 with standard ECL (WB)
expected | apparent MW		80.6 | 95 kDa (Arabidopsis thaliana)
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		dicots including: Glycine max, Nicotiana benthamina, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Sorghum bicolor, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea mays, trees: Populus balsamifera, moss: Physcomitrella patens, algae: Micromonas pulsilla, Ostreococcus lucimarinus
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		He et a. (2012). Specific Missense Alleles of the Arabidopsis Jasmonic Acid Co-Receptor COI1 Regulate Innate Immune Receptor Accumulation and Function. PloS Genetics, Open Access.

application example

5 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (C) or heat treated at 38ºC for 2 hours prior to protein extraction (HS). As positive control 10 ng of recombinant Hsp90-2 or Hsp90-1 were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 3000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602), diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and
treated with ECL detection reagent according to the manufacturer's instructions. Exposure time was 20 seconds.