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                                當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > TIP1;1,TIP1;2 | tonoplast intrinistic protein1-1, 1-2 (gamma)
                                TIP1;1,TIP1;2 | tonoplast intrinistic protein1-1, 1-2 (gamma)
                                英文名稱:TIP1;1,TIP1;2 | tonoplast intrinistic protein1-1, 1-2 (gamma)總訪問:527
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:8
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS09492 最后更新:2024-12-5
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                                product information
                                background

                                TIPs belong to MIP/aquaporin protein family. Alternative names: gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein

                                immunogen

                                KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. 2001)

                                antibody format

                                rabbit

                                polyclonal,

                                serum

                                quantity

                                100 µl

                                storage

                                store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

                                tested applications

                                ELISA (ELISA), western blot (WB)

                                related products

                                collection of antibodies to tonoplast proteins

                                AS09 482 | anti-AtTIP1;1 | gamma TIP

                                AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

                                AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

                                AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

                                AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

                                additional information

                                0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

                                Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

                                Protocol for isolation of vacuolar membranes can be found here.

                                application information
                                recommended dilution

                                1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

                                expected | apparent MW

                                25.8| 23 kDa

                                confirmed reactivity

                                Arabidopsis thaliana, Raphanus sativus

                                predicted reactivity

                                dicots including: Brassica napus

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

                                Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

                                Manufactured by Operon Biotechnologies.

                                selected references

                                Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs. Planta 212:294-304.


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                                application example

                                1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP1s antibodies (AS09 492, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

                                western blot using anti-TIP1s antibodies
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