| product information
| | background | | Ferritin is the major non-toxic iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. | | immunogen | | KLH-conjugated synthetic peptide derived from known plant ferritin sequences including Arabidopsis thaliana Q39101, At5g01600 | | antibody format | | rabbit, | polyclonal | | serum, | lyophilized | | | quantity | | 200 µl | - for reconstitution add 200 µl of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | AS06 126 | anti-Fer1 | ferritin 1 (pre-apoferritin), algal antibody
| | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1:3000 with standard ECL (WB) |
| expected | apparent MW | | 28 | 30 kDa
|
| confirmed reactivity | | Arabidopsis thaliana
|
| predicted reactivity | | dicots: Phaseolus vulgaris, Solanum tuberosum, Ricinus communis, monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea mays, moss: Physcomitrella patens
|
| not reactive in | | Ostreococcus tauri |
| additional information | | antibody is recognizing a chloroplastic form of plant ferritin |
| selected references | | to be added when available, antibody released in June 2011
|
application example
10 µg of total protein from Arabidopsis thaliala whole seedlings, various cellular fractions, extracted with (see below*) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed four times for 5 min inPBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 180 seconds.
*Following is the protein extraction buffer composition that was used to extract the total protein from the whole seedlings:50mM Tris, 4% SDS, 1% PVPP , 5% Glycerol, 1mM PMSF, 2mM Pefabloc.
Courtesy of Anshika Jain and Erin Connolly, University of South Carolina, USA
bio-equip.com
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