product information

background
 

Chromogranin A (a protein of 457 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent   marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

immunogen
 

purified full length native protein P10645

antibody format
 

hen;

polyclonal;

total IgY in 0.9 % NaCl, 0.1 % sodium azide;

liquid

quantity
 

200 µl

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB), ELISA (ELISA)

related products
 

to be added when available

additional information
 

IgY fraction at 12.2 mg/ml. IgY fraction was isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information

recommended dilution
 

1: 1000 - 1: 5000 with standard ECL (WB), 1: 1000 - 1: 5000 (ELISA)

expected | apparent MW
 

51 kDa

confirmed reactivity
 

human

predicted reactivity
 

human

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

antibodies are specific against chromogranin A when tested in immunoblotting against urine from patients with carcinoid tumours

selected references
 

to be added when available

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