product information

background
 

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains, each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

immunogen
 

purified full length native protein P02787

antibody format
 

hen;

polyclonal;

affinity purified IgY at 1mg/ml in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1 % sodium azide, pH 7.2;

liquid

quantity
 

200 µl (1 µg/µl)

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB)

related products
 

IMS02-020-322 | TF | Transferrin, hen antibody

AS10 1370 | TF | Transferrin, goat antibody

additional information
 

to be added when available

application information

recommended dilution
 

1: 5000 (WB)

expected | apparent MW
 

77 kDa

confirmed reactivity
 

human

predicted reactivity
 

primates

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum, dilution 1:10, or human transferrin. Only one precipitin arc in immunoelectrophoresis against normal human serum, or human transferrin. Specific against human transferrin in electroblotting when tested against normal human serum.

selected references
 

to be added when available

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