product information

background
 

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.

immunogen
 

human and rat alpha-1-microglobulin, native, full length, purified from urine

antibody format
 

mouse;

monoclonal;

IgG2a;

lyophilized

quantity
 

100 µg

- for reconstitution add 200 µl of sterile water

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB), ELISA (ELISA), immunoprecipitation (IP), radioimmunoassay (RIA), immunohistochemistry (IHC), affinity chromatography (ACH), immunoflourescence (IF)

related products
 

AS01 014 | anti-rat alpha-1 microglobulin

additional information
 

antibody is purified and contains 0.5 mg/ml of mouse albumin in 150 mM NaCl, 20 mM Tris-HCl, pH 8 and 0.02% sodium azide;

application information

recommended dilution
 

1: 2000 (WB), 0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF)

expected | apparent MW
 

27 kDa

confirmed reactivity
 

human, guinea pig, rabbit, rat

predicted reactivity
 

to be added when available

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

for usage on rabbit and guinea pig the antibody has to be applied in high concentrations.

The antibody has been used on frozen sections only, not paraffin.  Suggested antigen retrieval is 5 min in microwave owen in citrate buffer, pH 6.

selected references
 

Olsson et al. (2008). The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic. Res. 42:725-732; Yokota et al. (2007) Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics, 6:738-44.

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