product information

background
 

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

immunogen
 

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

antibody format
 

rabbit

polyclonal

serum,

lyophilized

quantity
 

200 µl,

for reconstitution add 200 µl, of sterile water.

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

ELISA (ELISA)

related products
 

AS05 087-20 | anti-GnRHa, smaller unit size of AS05 087

AS05 088 | anti-GnRHa hen antibody

additional information
 

to be added when available

application information

recommended dilution
 

1: : 80 000 (ELISA)

expected | apparent MW
 

n.a.

confirmed reactivity
 

GnRHa

predicted reactivity
 

GnRHa

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells. After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent.

selected references
 

Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297. 

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