product information

background
 

Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. It  occurs as dimer of two 200 kDa subunits. Fibronectin has binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

immunogen
 

purified, full length native protein P02751

antibody format
 

hen;

polyclonal;

affinity purified IgY at 1 mg/ml in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2;

liquid

quantity
 

200 µl

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB)

related products
 

IMS02-060-314 | anti-Fibronectin

additional information
 

affinity purified IgY on human fibronectin agarose

application information

recommended dilution
 

1: 5 000 with standard ECL (WB)

expected | apparent MW
 

263 kDa

confirmed reactivity
 

human

predicted reactivity
 

bovine, mouse

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

before labelling: only one precipitin arc in immunodiffusion against normal human serum or human fibronectin. Only one precipitin arc in immunoelectrophoresis against normal human serum or human fibronectin. Specific in immunoblotting against human fibronectin

selected references
 

to be added when available

---

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com

Save as PDF