product information

background
 

 40S ribosomal protein S14-1 is localized in cytoplasm and belongs to ribosomal protein S11P family.

immunogen
 

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana  Q9SIH0

antibody format
 

rabbit

polyclonal,

serum,

quantity
 

100 µl

storage
 

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications
 

ELISA (ELISA), western blot (WB)

related products
 

AS09 478 | anti-L13-1 | 60S ribosomal protein L13-1

additional information
 

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well.

application information

recommended dilution
 

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

expected | apparent MW
 

16 | 15 kDa

confirmed reactivity
 

Arabidopsis thaliana

predicted reactivity
 

dicots including: Brassica napus, Lupinus luteus, Ricinus communis,  monocots including: Oryza sativa, Zea mays,  trees: Picea sitchensis, Populus trichocarpa, algae: Chlamydomonas reinhardii, microalgae: Ostreococcus tauri

other species: chicken, human, mouse, rat, salmon, Candidia albicans, Trypanosoma brucei,

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnolgies.

selected references
 

to be added when available

application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-S14 antibodies (AS09 473, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

 

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