product information

background
 

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic

immunogen
 

KLH-conjugated synthetic peptide, amino acids 43-57 derived from human SOD1 sequence P00441

antibody format
 

rabbit

polyclonal,

serum,

lyophilized

quantity
 

50 µl

- for reconstitution add 50 µl of sterile water

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

western blot (WB)

related products
 

AS09 534 | SOD1 aa 03-20 | superoxide dismutase 1, soluble

AS09 535 | SOD1 aa 24-39 | superoxide dismutase 1, soluble

AS09 537 | SOD1 aa 58-72 | superoxide dismutase 1, soluble

AS09 538 | SOD1 aa 80-96 | superoxide dismutase 1, soluble

AS09 539 | SOD1 aa 100-115 | superoxide dismutase 1, soluble

AS09 540 | SOD1 aa 131-153 | superoxide dismutase 1, soluble

additional information
 

to be added when available

application information

recommended dilution
 

1 : 75 000 with standar ECL (WB)

expected | apparent MW
 

15.9 kDa

confirmed reactivity
 

human

predicted reactivity
 

chimpanzee, fission yeast

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

Peptide used to elicit this antibody is not conserved in SOD2,3 and 4.

selected references
 

antibody available in October 2009

application example 

 

 

 10 µl of control mouse brain and a human spinal ventral horn were extracted in 25 volumes of PBS, diluted 1+1 in a sample buffer have been loaded per well and separated on 15% SDS-PAGE and blotted for 1h to PVDF. Blots were blocked immediately following transfer in 5% Dry milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 75 000 (AS09 536) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, from DAKO PO448) diluted to 1:42 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager. Exposure time was 30-600 seconds.

 

 

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