product information

background
 

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1,   HSP70-1/HSP70-2,  Heat shock 70 kDa protein 8

immunogen
 

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142

antibody format
 

rabbit;

polyclonal;

affinity purified serum in PBS pH 7.4;

lyophilized;

quantity
 

100 µg

- for reconstitution add 100 µl of sterile water

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB), immunoprecipiation (IP)

related products
 



additional information
 

for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371

application information

recommended dilution
 

1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP)

expected | apparent MW
 

70 kDa

confirmed reactivity
 

fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Gloeophyllum protractum, G. sepiarium, G. carbonarium, Oligoporus sericiomollis, Phlebia cornea, Junghunia luteoalba

predicted reactivity
 

vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

this antibody is not suitable for work with samples from higher plants

selected references
 

to be added when available

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