The melanin-concentrating hormone, also known as MCH and Pro-MCH, is a cyclic neuropeptide that belongs to the melanin-concentrating hormone family. It is isolated initially from salmon pituitary gland and later from rat hypothalamus. MCH predominantly expressed in lateral hypothalamus, also detected in pallidum, neocortex and cerebellum. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal.It may also have a role in spermatocyte differentiation.

·Description
Novoprotein Recombinant Human Pro-MCH/PMCH produced by mammalian cells expression system.Target protein is expressed with sequence (Ile22-Val165) of Human pmch (Uniprot #P20382) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of Pro-MCH/PMCH (Human)

·Formulation
  Novoprotein Recombinant Human PMCH was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human PMCH in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human PMCH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human PMCH should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.