Peptidyl-prolyl cis-trans isomerase B, also known as PPIase B, Rotamase B, Cyclophilin B, CYPB, and PPIB, is a member of the cyclophilin-type PPIase family and PPIase B subfamily which contains one PPIase cyclophilin-type domain. Cyclophilin B/CYPB is a cyclosporin A binding proteins that can be secreted in response to inflammatory stimuli. Cyclophilin B interaction with prolactin potentiated prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin. The intranuclear prolactin/cyclophilin B complex acts as a transcriptional inducer by interacting directly with Stat5, resulting in the removal of the Stat-repressor protein inhibitor of activated Stat 3 (PIAS3), thereby enhancing Stat5 DNA-binding activity and prolactin-induced, Stat5-mediated gene expression. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9).

·Description
Novoprotein Recombinant Human Cyclophilin B produced by mammalian cells expression system.Target protein is expressed with sequence (Asp34-Glu216) of Human cyclophilin b (Uniprot #P23284) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of Cyclophilin B (Human)

·Formulation
  Novoprotein Recombinant Human PPIB was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human PPIB in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human PPIB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human PPIB should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.