Proteinase-3, also called PR-3, Neutrophil proteinase 4, NP-4, Myeloblastin, AGP7 and C-ANCA antigen, is a neutral serine proteinase which belongs to the peptidase S1 family and Elastase subfamily. It contains one peptidase S1 domain and expresses mainly in neutrophil granulocytes. The primary function of Proteinase3 is thought to be degradation of extracellular proteins at sites of inflammation, but excessive or prolonged proteolytic activity may cause harmful effects in the body. It is the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.

·Description
Novoprotein Recombinant Human Proteinase 3/Myeloblastin/PRTN3 produced by mammalian cells expression system.Target protein is expressed with sequence (Ala26-Arg249) of Human proteinase 3 (Uniprot #P24158) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of Proteinase 3/Myeloblastin/PRTN3 (Human)

·Formulation
  Novoprotein Recombinant Human Proteinase 3 was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human Proteinase 3 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human Proteinase 3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human Proteinase 3 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.