Roundabout homolog 1, also named ROBO1, Deleted in U twenty twenty and H-Robo-1, is a single-pass type I membrane protein which belongs to the neural adhesion molecule (NCAM) family of receptors. ROBO1 contains 3 fibronectin type-III domains and 5 Ig-like C2-type (immunoglobulin-like) domains. It is widely expressed, with exception of kidney. It is a receptor for SLIT1 and SLIT2 which are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Defects in Roundabout homolog 1 may be a cause of breast and lung cancer. ROBO1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line.

·Description
Novoprotein Recombinant Human ROBO1 produced by mammalian cells expression system.Target protein is expressed with sequence () of Human robo1 (Uniprot #B2RXI1) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of ROBO1 (Human)

·Formulation
  Novoprotein Recombinant Human ROBO1 was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human ROBO1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human ROBO1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human ROBO1 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.