Semaphorin-4G is the least characterized of seven known Class 4 transmembrane semaphorin glycoproteins. Class 4 semaphorins play multiple roles in cell attraction or repulsion, such as development of nerve pathways in the brain, promoting or inhibiting proliferation, in some cases organizing immune cell interactions. Semaphorin-4G can be expressed early in development in the central and peripheral nervous systems and in sensory ograns, such as cochlea, olfactory epithelium, vomeronasal organ and retina. In aults, Semaphorin-4G can be found in liver,kidney and brain.The human Semaphorin-4G precursor consists of a 17 amino acids signal sequence, a 658 amino acids extracellular domain, a 21 amino acids transmembrane domain, a 142 amino acids cytoplasmic domain with one Ser/Thr phosphorylation site.

·Description
Novoprotein Recombinant Human Semaphorin 4G produced by mammalian cells expression system.Target protein is expressed with sequence (Val18-Leu680) of Human semaphorin 4g (Uniprot #Q9NTN9) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of Semaphorin 4G (Human)

·Formulation
  Novoprotein Recombinant Human SEMA4G was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human SEMA4G in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human SEMA4G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human SEMA4G should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.