Kunitz-type protease inhibitor 2 , also known as HAI-2 , SPINT2 and KOP, is a Kunitz-type serine protease inhibitor. SPINT2 is a single-pass type I membrane protein and contains two BPTI/Kunitz inhibitor domains. the first Kunitz domain is mainly responsible for the inhibitory activity against hepatocyte growth factor activator (HGFA). SPINT2 is expressed in placenta, kidney, pancreas, prostate, testis, thymus and trachea. SPINT2 serves as a inhibitor of HGF activator. It also inhibits plasmin, plasma and tissue kallikrein and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3), also known as congenital sodium diarrhea (CSD).

·Description
Novoprotein Recombinant Human HAI-2 produced by mammalian cells expression system.Target protein is expressed with sequence (Ala28-Lys197) of Human hai-2 (Uniprot #O43291) fused with a polyhistidine tag at the C-terminus.

·Quality Control

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 

Endotoxin:Less than 0.1ng/μg (1EU/μg) of HAI-2 (Human)

·Formulation
  Novoprotein Recombinant Human SPINT2 was lyophilized at 1mg/ml in 20mM PBS.

·Reconstitution
We recommend a quick spin followed by reconstitution of Novoprotein Recombinant Human SPINT2 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to working solutions.

·Storage
Lyophilized Recombinant Human SPINT2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant Human SPINT2 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.