CD58, also known as lymphocyte function associated antigen (LFA3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 has only one known ligand, CD2, which is expressed on T cells, NK cells and dendritic cells. CD58/CD2 interaction is involved in T cell costimulation and is important in mediating T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity.

·Description
Recombinant Human CD58 produced by transiently transfected human cell is the extracellular domain (Accession#p19256)(Phe29-Arg215) of human CD58 fused with a polyhistidine tag at the C-terminus.

·Quality Control

Biological activity:
The ED50 of recombinant CD58 in a cell proliferation assay using human CD3+ T cells is 1-4μg/mL in the presence of antiCD3 immobilized at 20 ng/mL..

Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Phe29.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHu CD58.

·Formulation
Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized rHu CD58 in sterile water not less than 100μg/ml.

·Storage
Lyophilized rHuCD58 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuCD58 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use..

Please avoid freeze-thaw cycles.