CD150, also known as Signaling Lymphocytic Activation Molecule (SLAM), is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 family of receptors. CD2 family proteins function as adhesion molecules and modulators of the immune response. The expression of CD150 is mainly restricted to immune cells. It consists of an extracellular domain (ECD) with two Ig like domains and a cytoplasmic domain with three SH2-binding motifs.As a self-ligand, CD150 performs diverse immunologic functions, promoting the activation of Th0/Th1 cells, B cells, eosinophils, mast cell, and macrophages. In humans, CD150 also functions as a cellular entry receptor for measles virus.

·Description
Recombinant Human SLAMF1/CD150 produced by transiently transfected human cell is the extracellular domain(Accession # Q13291) ( Ala21 – Pro237 ) of human SLAMF1/CD150 fused with a polyhistidine tag at the C-terminus.

·Quality Control
Purity: Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: Predicted N terminal amino acid is Ala21.
Endotoxin: Less than 0.1ng/μg (1EU/μg) of rHuCD150.

·Formulation
Sterile and Lyophilized from a 0.2 μm filtered solution in PBS.

·Reconstitution
It is recommended to reconstitute the lyophilized recombinant Human CD106 in sterile water at no less than 100 μg/ml.

·Storage
Lyophilized rHuCD150 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuCD150 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.

Please avoid freeze-thaw cycles.