Human Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family. CCL23 is also known as myeloid progenitor inhibitory factor MPIF-1, CK8 and SCYA23. CCL23 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CCL23 (aa 22 -120).Additional N-terminal Processing of the 99 aa residue variant can generate a 75 aa residue peptide (aa 46-120) that is significantly more active than the 99 aa residue variant.

CCL23 binds to CCR1 with high affinity and has chemotactic activity for monocytes,dendritic cells, and osteoclast precursors. CCL23 enhances angiogenesis of endothelial cells, but reduces the proliferation of progenitor cells giving rise to granulocyte and monocyte lineages.

·Description
Recombinant Human CCL23 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 100 amino acids with an extra N-terminal Met and having a molecular mass of 11.5kDa.

·Quality Control
Biological activity: The ED50 of rhCCL23 was 0.2-0.5 μg/mL as determined by its ability to chemoattract THP1 human acute monocytic leukemia cells.The ED50 of rhCCL23 was 0.02-0.1 μg/mL as determined by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Val-Thr-Lys.
Endotoxin: Less than 0.1ng/μg (1EU/μg) of rHuCCL23 as determined by LAL test.

·Formulation
Sterile and Lyophilized aliquots with a 0.2um filtered protein solution containing 50mM Tris-HCl pH8.0,500mM NaCl.

·Reconstitution
It is recommended to reconstitute the lyophilized rhCCL23 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other solutions.

·Storage
Lyophilized rHuCCL23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuCCL23 should be stored at 4°C between 2-7 days and aliquots of reconstituted samples are stable up to 3 months below -18°C for future use.


Please avoid freeze-thaw cycles.