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                                                              梅里埃 30526大腸桿菌免疫濃縮O157試劑盒
                                                              英文名稱:VIDAS® Immuno-Concentration E. coli O157 (ICE)總訪問:254
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:法國(guó)梅里埃產(chǎn)品類別:生化試劑
                                                              規(guī)       格:30T/盒 最后更新:2024-12-24
                                                              貨       號(hào):30526
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                                                              VIDAS® Immuno-Concentration E. coli O157 (ICE)
                                                              For microbiological control only
                                                              VIDAS® Immuno-Concentration E. coli O157 (ICE) is an automated test for use on the VIDAS® family of instruments, for
                                                              the selective enrichment of E. coli O157.
                                                              SUMMARY AND EXPLANATION
                                                              VIDAS® Immuno-Concentration E. coli O157 is intended
                                                              for use in conjunction with the VIDAS® UP E. coli O157
                                                              (including H7) (ECPT) assay. It enables the simplification
                                                              and easy interpretation of confirmation tests on samples
                                                              in which E. coli O157 have been detected by VIDAS®
                                                              ECPT.
                                                              Direct plating of certain enriched samples which screened
                                                              positive with VIDAS® ECPT can result in the overgrowth
                                                              of the target E. coli O157 by competitive enteric flora.
                                                              VIDAS® Immuno-Concentration E. coli O157 (ICE) is a
                                                              fully automated method for the selective concentration of
                                                              E. coli O157 from enrichment broth. The procedure
                                                              enhances the performance of the selective isolation
                                                              media (1).
                                                              For the sample types, please refer to the VIDAS® ECPT
                                                              (Ref. 30122) package insert.
                                                              PRINCIPLE
                                                              The VIDAS® Immuno-Concentration E. coli O157 (ICE) kit
                                                              includes two ready-to-use components:
                                                               A pipette tip-like disposable device, the Solid Phase
                                                              Receptacle (SPR® ), which serves as the solid phase as
                                                              well as the pipetting device for the assay. The interior of
                                                              the SPR® is coated with anti-E coli O157 antibodies
                                                              adsorbed on its surface.
                                                               A strip which contains all the wash and release
                                                              solutions.
                                                              Part of the enrichment broth is dispensed into the reagent
                                                              strip and the sample is cycled in and out of the SPR® .
                                                              E. coli O157 present in the broth will bind to the anti
                                                              E. coli O157 antibodies coating the interior of the SPR® .
                                                              Unbound sample components are then washed away.
                                                              A final enzymatic step releases the captured E. coli O157
                                                              into one of the strip wells.
                                                              The entire Immuno-concentration process is performed
                                                              automatically by the instrument.
                                                              CONTENT OF THE KIT (30 tests)
                                                              30 ICE strips STR Ready-to-use.
                                                              30 ICE SPRs SPR Ready-to-use.
                                                              Interior of SPR® s coated with antibodies directed against E coli O157-specific antigens.
                                                              ICE standard
                                                              (1 x 6 ml)
                                                              S1 Ready-to-use.
                                                              Alkaline phosphatase-labeled antibodies + preservative + protein stabilizer.
                                                              MLE data indicate the confidence interval in Relative Fluorescence Value ("Standard
                                                              (S1) RFV Range").
                                                              Specifications for the factory master data required to calibrate the test:
                                                               MLE data (Master Lot Entry) provided in the kit.
                                                              or
                                                               MLE bar code printed on the box label.
                                                              1 Package insert downloadable from www.biomerieux.com/techlib
                                                              The SPR
                                                              The SPR® is coated during production with antibodies
                                                              directed against E coli O157-specific antigens.
                                                              Each SPR is identified by the "ICE" code. Only remove
                                                              the required number of SPR® s from the pouch and
                                                              carefully reseal the pouch after opening.
                                                              The strip
                                                              The strip consists of 10 wells covered with a labeled, foil
                                                              seal. The label comprises a bar code which mainly
                                                              indicates the assay code, kit lot number and expiration
                                                              date.
                                                              The foil of the fourth well is perforated to facilitate the
                                                              introduction of the sample.
                                                              The first well, which is empty, is used to recover the
                                                              immuno-concentrated suspension.
                                                              The wells in the center section of the strip contain the
                                                              various reagents required for the assay.
                                                              bio-equip.com
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