Description:

Rabbit polyclonal antibody

  Background:

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. It is encoded by a multigene family of three members (At5g08670, At5g08680, At5g08690). F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c (9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.

  Synonyms:

AtpB, ATP synthase F1 sector subunit beta, F-ATPase subunit beta.

  Immunogen:

KLH-conjugated synthetic peptide of AtpB derived from Arabidopsis thaliana AT5G08670, AT5G08680, AT5G08690.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

60 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Chlamydomonas reinhardtii, Solanum tuberosum, Solanum lycopersicum, Nicotiana tabacum, Cucumis sativus, Glycine max, Vitis vinifera, Oryza sativa Japonica Group, Brassica napus, Brassica rapa, Zea mays, Triticum aestivum, Sorghum bicolor, Gossypium raimondii, Synechocystis sp. PCC 6803. 
The sequence of the synthetic peptide used for immunization is also 93% homologues with the sequence in chloroplast ATP synthase beta subunit ATCG00480.
For more species homologues information, please contact tech support at tech@phytoab.com.