Description:

Rabbit polyclonal antibody

  Background:

ATPase alpha subunit, which is a subunit of ATP synthase and part of the CF1 portion which catalyzes the conversion of ADP to ATP using the proton motive force. This complex is located in the thylakoid membrane of the chloroplast.

  Synonyms:

AtpA, ATP synthase F1 sector subunit alpha, F-ATPase subunit alpha

  Immunogen:

Recombinant full length ATP synthase alpha subunit of Arabidopsis thaliana ATCG00120.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

55 / 59 kDa(Arabidopsis thaliana), 55 kDa(Synechocystis sp. PCC 6803)

  Confirmed Reactivity: 

Arabidopsis thaliana, Synechocystis sp. PCC 6803

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.