當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 細(xì)胞生物學(xué)試劑 > PDGF BB Human

PDGF BB Human

英文名稱：	PDGF BB Human	總訪問：	305
國產(chǎn)/進(jìn)口：	進(jìn)口	半年訪問：	3
產(chǎn)地/品牌：	prospec	產(chǎn)品類別：	細(xì)胞生物學(xué)試劑
規(guī) 格：	CYT-501	最后更新：	2024-12-5
貨號：
CAS 號：
參考報(bào)價(jià)：	詢價(jià)
立即詢價(jià) 電話咨詢

[發(fā)表評論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏]

分享：微信新浪微博

銷售商：北京啟維益成科技有限公司

查看該公司所有產(chǎn)品 >>

產(chǎn)品介紹
公司簡介

Catalogue Number

CYT-501

Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.

Introduction

PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

Description

Platelet-Derived Growth Factor BB Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10mM Acetic Acid.

Solubility

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in 100mM Acetic Acid.

Stability

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1ng/ml, corresponding to a Specific Activity of 1MIU/mg.

Protein content

Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.

References

1.Title:Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.
Publication:Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol. 115 no. 1 51-60 .
Link:http://bloodjournal.hematologylibrary.org/content/115/1/51.full

2.Title:Imatinib attenuates skeletal muscle dystrophy in mdx mice.
Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548.
Link:http://www.fasebj.org/content/23/8/2539.full

2.Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing .
Publication:

Cell Transplantation

ISSN: 0963-6897

DOI: 10.3727/096368910X516637

Volume 19, Issue 12, pages 1635-1644

Link:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000012/art00011

Usage

ProSpec's products are furnishe>

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗(yàn)人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時(shí)俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國，讓國內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。

我們代理的產(chǎn)品覆蓋細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。

細(xì)胞生物學(xué)包含細(xì)胞培養(yǎng)，細(xì)胞遷移，細(xì)胞信號轉(zhuǎn)導(dǎo)等。

植物學(xué)包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。

分子生物學(xué)包含表觀遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。

免疫學(xué)包含抗體，各類檢測試劑盒等。

生物化學(xué)包含各類生化試劑，化學(xué)小分子，常規(guī)試劑等。

蛋白組學(xué)包含不同種屬的重組蛋白，天然提取蛋白，細(xì)胞因子等。

生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。

在公司全體員工的共同努力和國外生產(chǎn)商的支持下，在業(yè)務(wù)上實(shí)現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長春、石家莊、哈爾濱等重點(diǎn)城市建立了分支機(jī)構(gòu)或授權(quán)服務(wù)商，并以專業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強(qiáng)企業(yè)的中國區(qū)產(chǎn)品供應(yīng)商，以及中國科學(xué)院科研采購平臺的中標(biāo)供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進(jìn)了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷售平臺，業(yè)務(wù)代表銷售平臺，針對科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡(luò)平臺和業(yè)務(wù)代表平臺的優(yōu)點(diǎn)，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨(dú)立的營銷團(tuán)隊(duì)，分別配備不同的專業(yè)技術(shù)支持和客服人員，通過網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。

公司客戶遍布中國生命科學(xué)基礎(chǔ)研究、開發(fā)應(yīng)用、制藥、疾病診斷與控制、商品檢驗(yàn)檢疫、生物技術(shù)公司、食品安全以及農(nóng)林畜牧業(yè)等諸多領(lǐng)域。

我們追求目的是與研究機(jī)構(gòu)及合作伙伴之間誠信而專業(yè)的對話，力求建立長久合作。我們相信我們精心提供的高效、優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務(wù)，會使您實(shí)驗(yàn)設(shè)計(jì)更有效，實(shí)驗(yàn)操作更簡便，研究結(jié)果更可靠。

放眼未來，公司將努力實(shí)現(xiàn)更大的突破，將國外優(yōu)秀產(chǎn)品不斷引進(jìn)國內(nèi)，同時(shí)積極推動國內(nèi)的產(chǎn)品銷往世界各地

售后服務(wù)

相關(guān)視頻

資料下載

快速詢價(jià) 請登錄或注冊在線詢價(jià)　(請留下您的聯(lián)系方式，以便供應(yīng)商聯(lián)系您)