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PLGF 1 Human
英文名稱：PLGF 1 Human總訪問：188
國產(chǎn)/進口：進口半年訪問：1
產(chǎn)地/品牌：prospec產(chǎn)品類別：細胞生物學試劑
規(guī)       格：CYT-419 最后更新：2024-12-5
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Catalogue Number CYT-419
Synonyms PIGF, PGF, PLGF-1, PlGF-2.
Introduction PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.
Description Placenta Growth Factor-1 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa.
The PLGF-1 is purified by proprietary chromatographic techniques.
Source Insect Cells.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Each mg of contains 50mg BSA.
Solubility It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.
Biological Activity The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5-10ng/ml corresponding to a Specific Activity of 0.1-2MIU/mg.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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