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Myostatin Human, Plant
英文名稱(chēng):Myostatin Human, Plant總訪問(wèn):241
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產(chǎn)地/品牌:prospec產(chǎn)品類(lèi)別:細(xì)胞生物學(xué)試劑
規(guī)       格:CYT-055 最后更新:2024-12-5
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Catalogue Number CYT-055
Synonyms GDF-8, MSTN, Growth Differentiation Factor 8, MSTN Muscle Hypertrophy.
Introduction GDF8 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth.
Description Myostatin Human Recombinant produced in Nicotiana benthamiana plant is a single chain containing 115 amino acids (molecular formula C586H865N165O164S12) and 6-His-tag at the N-terminal having the total molecular mass of 13.2kDa.
Source Nicotiana benthamiana plant
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from 1mg/ml solution in glycine 0.05M buffer at pH 8.5 and 100mM NaCl.
Solubility It is recommended to reconstitute the lyophilized Myostatin in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Myostatin should be stored at 4°C between
2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 97.0% as determined by Analysis by SDS-PAGE.
Amino acid sequence HHHHHHDFGL DCDEHSTESR CCRYPLTVDF EAFGWDWIIA PKRYKANYCS GECEFVFLQK
YPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII YGKIPAMVVD RCGCS
Serological Identification The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Myostatin/ antiHis antibody (reducing conditions ~13kDa monomer and ~26kDa homodimers).
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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