|
||||||||||||||||||||||||||||||||||
[發(fā)表評論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏] |
銷售商: 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
Catalogue Number | CYT-464 |
Synonyms | IGFBP-5, IBP-5, IGF-binding protein 5. |
Introduction | IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Description | IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. IGFBP5 is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate PH 3.0. |
Solubility | It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe. |
Biological Activity | The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15ng/ml of Human IGF-II corresponding to a Specific Activity of 66,667IU/mg. |
Usage | ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. |