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PIXY321 Human
英文名稱：PIXY321 Human總訪問：303
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產(chǎn)地/品牌：prospec產(chǎn)品類別：細胞生物學試劑
規(guī)       格：CYT-360 最后更新：2024-12-5
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Catalogue Number CYT-360
Synonyms PIXY321, GMCSF/ IL3.
Introduction Synergism of IL3 together with GMCSF causes a rapid rise in neutrophils due to GMCSF and a slower rise in platelets due to IL3 which has strong stimulated effect on hematopoietic cells.
The analog of GMCSF/IL3 is PIXY321. Results indicate that PIXY321 can stimulate multilineage hematopoiesis in vivo and enhance neutrophil and platelet recovery following chemotherapy and bone marrow transplantation (BMT). These results suggest that PIXY321 elicits the biological effects of both its component cytokines and represents a novel means of delivering two independent but interactive cytokines in combination.
Description GMCSF/IL3 fusion protein Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kDa.
PIXY321 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized after extensive dialysis against 10mM PBS, pH= 7.4±0.1 and 0.1% BSA.
Solubility It is recommended to reconstitute the lyophilized PIXY321 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PIXY321should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by1. Analysis by RP-HPLC.
2. Analysis by SDS-PAGE.
Biological Activity The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.2ng/ml, corresponding to a Specific Activity of 5MIU/mg.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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