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GM CSF Mouse

英文名稱：	GM CSF Mouse	總訪問：	237
國產(chǎn)/進口：	進口	半年訪問：	1
產(chǎn)地/品牌：	prospec	產(chǎn)品類別：	細胞生物學(xué)試劑
規(guī) 格：	CYT-222	最后更新：	2024-12-5
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Catalogue Number

CYT-222

Synonyms

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.

Introduction

GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Description

Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.
GM-CSF Mouse is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

GM-CSF Mouse was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

Protein content

GM-CSF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.

References

1.Title:Multigene/multisubtype HIV-1 vaccine induces potent cellular and humoral immune responses by needle-free intradermal delivery.
Publication: Mol Ther. 2005 Dec;12(6):1197-205. Epub 2005 Aug 22. PMID: 16112909
Link: http://www.nature.com/mt/journal/v12/n6/full/mt20051405a.html
Applications: The Mouse GM-CSF used for 2 purposes:
1. As an adjuvant for DNA vaccine which included seven plasmids encoding nine HIV-1 proteins. The mice were injected with the DNA vaccine together with recombinant mouse GM-CSF. 2. Used in Elisa.

2.Title: Neospora caninum: cloning and expression of a gene coding for cytokine-inducing profilin.
Publication: Exp Parasitol. 2010 Aug;125(4):357-62. Epub 2010 Mar 6. PMID: 20211619
Link: http://www.sciencedirect.com/science/article/pii/S001448941000086X
Applications: Used for immunoblotting

3.Title: Intranasal Granulocyte-Macrophage Colony-Stimulating Factor Reduces the Aspergillus Burden in an Immunosuppressed Murine Model of Pulmonary Aspergillosis.
Publication: Antimicrob Agents Chemother. 2008 February; 52(2): 716–718.
Published online 2007 November 5
Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2224773/?tool=pmcentrez
Applications: GM-CSF as tested a ther>

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時俱進地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標是把國外有特點的生物前沿產(chǎn)品引進中國，讓國內(nèi)科學(xué)研究機構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。

我們代理的產(chǎn)品覆蓋細胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。

細胞生物學(xué)包含細胞培養(yǎng)，細胞遷移，細胞信號轉(zhuǎn)導(dǎo)等。

植物學(xué)包含植物抗體，植物抗生素，植物組織培養(yǎng)試劑等。

分子生物學(xué)包含表觀遺傳試劑盒，核酸提取，轉(zhuǎn)染試劑等。

免疫學(xué)包含抗體，各類檢測試劑盒等。

生物化學(xué)包含各類生化試劑，化學(xué)小分子，常規(guī)試劑等。

蛋白組學(xué)包含不同種屬的重組蛋白，天然提取蛋白，細胞因子等。

生物制藥與診斷試劑研發(fā)方面的試劑包含各類蛋白抑制劑，酶類等。

在公司全體員工的共同努力和國外生產(chǎn)商的支持下，在業(yè)務(wù)上實現(xiàn)了迅猛發(fā)展，相繼在上海、武漢、廣州、長春、石家莊、哈爾濱等重點城市建立了分支機構(gòu)或授權(quán)服務(wù)商，并以專業(yè)和優(yōu)質(zhì)的服務(wù)成為多家世界500強企業(yè)的中國區(qū)產(chǎn)品供應(yīng)商，以及中國科學(xué)院科研采購平臺的中標供貨商。2008年以后業(yè)務(wù)的迅猛發(fā)展促進了啟維益成內(nèi)部組織架構(gòu)的不斷優(yōu)化，我們建立了自己的網(wǎng)絡(luò)銷售平臺，業(yè)務(wù)代表銷售平臺，針對科研客戶與企業(yè)客戶的不同需求，綜合網(wǎng)絡(luò)平臺和業(yè)務(wù)代表平臺的優(yōu)點，啟維益成創(chuàng)建了科研用戶與工業(yè)客戶兩支獨立的營銷團隊，分別配備不同的專業(yè)技術(shù)支持和客服人員，通過網(wǎng)絡(luò)和業(yè)務(wù)代表，力求為不同類型的客戶提供前沿專業(yè)的解決方案。

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